Kirik I A, Babykin M M
Genetika. 2008 May;44(5):717-20.
The sll0886 gene, controlling light-activated heterotrophic growth (LAHG), was tested for the role in regulating phototaxis in cyanobacterium Synechocystis sp. PCC 6803. Insertional inactivation of the gene in the genome of a wildtype strain did not affect positive (toward light) or negative (away from high light) phototaxis. However, cells lost motility when sll0886 inactivation was combined with the prqRL17Q mutation, which determined negative phototaxis at low light. Immotile cells with the prqRL17Q mutation and the inactivated sll0886 gene did not display any defect in the formation of type IV pili, essential for phototaxis. Hence, the function, rather than biogenesis, of pili was affected. It was concluded that the sll0886 gene, coding for a TPR family protein, is involved in controlling negative phototaxis of cyanobacteria at the level of photoreception and signal transduction and that its role is mediated by the unidentified redundant gene whose function is suppressed by the prqRL17Q mutation.
对控制光激活异养生长（LAHG）的sll0886基因在调节蓝藻集胞藻PCC 6803趋光性中的作用进行了测试。在野生型菌株基因组中该基因的插入失活并不影响正向（向光）或负向（远离强光）趋光性。然而，当sll0886失活与prqRL17Q突变相结合时，细胞失去了运动性，prqRL17Q突变决定了在弱光下的负向趋光性。具有prqRL17Q突变和失活的sll0886基因的不运动细胞在IV型菌毛形成方面没有表现出任何缺陷，IV型菌毛是趋光性所必需的。因此，菌毛的功能而非生物发生受到了影响。得出的结论是，编码TPR家族蛋白的sll0886基因在光感受和信号转导水平上参与控制蓝藻的负向趋光性，并且其作用是由未鉴定的冗余基因介导的，该冗余基因的功能被prqRL17Q突变所抑制。
