Mascetti J, Castano S, Cavagnat D, Desbat B
ISM, Institut des Sciences Moleculaires (UMR 5255 CNRS), Universite Bordeaux 1, 351, cours de la Liberation, 33405 Talence Cedex, France.
Langmuir. 2008 Sep 2;24(17):9616-22. doi: 10.1021/la8004294. Epub 2008 Aug 2.
The complexation of beta-cyclodextrin with monolayers of cholesterol, DMPC, DMPG, and mixtures of those lipids has been studied using Brewster microscopy, PMIRRAS, and ab initio calculations. An oriented channel-like structure of beta-cyclodextrin, perpendicular to the air/water interface, was observed when some cholesterol molecules were present at the interface. This channel structure formation is the first step in the cholesterol dissolution in the subphase. With pure DMPC and DMPG monolayers, weaker, less organized complexes are formed, but they disappear almost completely at high surface pressure, and only a small amount of phospholipid is dissolved in the subphase.
已使用布鲁斯特显微镜、偏振调制红外反射吸收光谱法（PMIRRAS）和从头计算法研究了β-环糊精与胆固醇单层、二肉豆蔻酰磷脂酰胆碱（DMPC）、二肉豆蔻酰磷脂酰甘油（DMPG）以及这些脂质混合物的络合作用。当界面处存在一些胆固醇分子时，观察到β-环糊精形成了垂直于空气/水界面的定向通道状结构。这种通道结构的形成是胆固醇在亚相中溶解的第一步。对于纯DMPC和DMPG单层，会形成较弱、组织性较差的络合物，但在高表面压力下它们几乎完全消失，并且只有少量磷脂溶解在亚相中。
