通过共聚焦显微镜和图像分析对少突胶质细胞瘤的 GFAP 和 alpha1a-AR 染色及核形态计量学：预测少突胶质细胞瘤生存的有用参数。

GFAP and alpha1a-AR staining and nuclear morphometry of oligodendrogliomas by confocal microscopy and image analysis: useful parameters for predicting survival in oligodendrogliomas.

机构信息

Universidad Rey Juan Carlos, Faculty of Health Sciences, Av. Atenas s/n, Madrid, Spain.

出版信息

Diagn Pathol. 2008 Jul 15;3 Suppl 1(Suppl 1):S26. doi: 10.1186/1746-1596-3-S1-S26.

DOI:10.1186/1746-1596-3-S1-S26

PMID:18673515

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2500107/

Abstract

OBJECTIVE

This study attempts to evaluate the GFAP and alpha1a-AR staining and morphometrical nuclear features of oligodendrogliomas and their prognostic implications as compared to present histopathology classification and their survival outcome.

STUDY DESIGN

Surgical specimens from 24 patients with oligodendrogliomas during the period 1981-2000 were included. These cases were classified into two groups defined by the grade of the neoplasm: Group I: oligodendrogliomas grade II; Group II: oligodendrogliomas grade III and two groups based on the outcome status: Group of the alive cases and group of the death cases. Death rate for the groups were obtained by patients' charts. Descriptive statistics were used to examine the groups with respect to the morphometrical nuclear variables; area, perimeter, aspect, axes (major and minor), diameters (max, mean and min.), radius (max. and min.) margination, ratio of perimeter-area, roundness and sizes (length and width). In addition, an immunofluorescence method for GFAP and 1a-AR were performed and their area, density and intensity of staining were analyzed.

RESULTS

Semiautomated quantitative morphometrical results showed that the variables of nuclear area (GII 48.87 microm2 vs. GIII 43.45 microm2 p-value = 0.02), aspect (GII 1.39 vs. GIII 1.55 p-value = 0.03), axis minor (GII 6.66 microm vs. GIII 6.01 microm p-value = 0.003), diameter minor (GII 5.93 microm vs. GIII 5.27 microm p-value = 0.002), radius minor (GII 2.64 microm vs. GIII 2.25 microm p-value = 0,003), perimeter-area (GII 0.0007 vs. GIII 0.0006 p-value = 0.04), size width (GII 6.60 microm vs. GIII 5.96 microm p-value = 0,003), and density of alpha1a-AR staining (GII 121.38 vs. GIII 146.03 p-value = 0.05) were statistically significant in regard of grade; and that the sum of density of GFAP (p-value = 0.01) and the intensity of alpha1a-AR (p-value = 0.01) were statistically significant in predicting survival.

CONCLUSION

These results suggest that some nuclear morphometrical features and the GFAP and alpha1a-AR immunofluorescence staining may be useful parameters for predicting survival in oligodendrogliomas.

摘要

目的

本研究旨在评估寡突胶质细胞瘤中 GFAP 和 alpha1a-AR 染色及核形态计量学特征，并与目前的组织病理学分类及其生存结果进行比较，以评估其预后意义。

研究设计

纳入了 1981 年至 2000 年期间 24 例寡突胶质细胞瘤的手术标本。这些病例根据肿瘤分级分为两组：I 组：少突胶质细胞瘤 II 级；II 组：少突胶质细胞瘤 III 级；根据预后状态分为两组：存活组和死亡组。通过患者病历获得各组的死亡率。采用描述性统计方法，对各组核形态计量学变量（面积、周长、形态、轴（长轴和短轴）、直径（最大、平均和最小）、半径（最大和最小）、边缘、周长-面积比、圆度和大小（长和宽））进行了分析。此外，还进行了 GFAP 和 1a-AR 的免疫荧光法，并分析了它们的面积、密度和染色强度。

结果

半自动化定量形态计量学结果显示，核面积（GII 48.87 µm2 与 GIII 43.45 µm2，p 值=0.02）、形态（GII 1.39 与 GIII 1.55，p 值=0.03）、短轴（GII 6.66 µm 与 GIII 6.01 µm，p 值=0.003）、小直径（GII 5.93 µm 与 GIII 5.27 µm，p 值=0.002）、小半径（GII 2.64 µm 与 GIII 2.25 µm，p 值=0.003）、周长-面积比（GII 0.0007 与 GIII 0.0006，p 值=0.04）、宽度大小（GII 6.60 µm 与 GIII 5.96 µm，p 值=0.003）和 alpha1a-AR 染色密度（GII 121.38 与 GIII 146.03，p 值=0.05）在分级方面具有统计学意义；而 GFAP 密度总和（p 值=0.01）和 alpha1a-AR 强度（p 值=0.01）在预测生存方面具有统计学意义。