Nematbakhsh Mehdi, Haghjooyjavanmard Shaghayegh, Mahmoodi Farzaneh, Monajemi Ali Reza
Deparment of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Lipids Health Dis. 2008 Aug 2;7:27. doi: 10.1186/1476-511X-7-27.
The impact of L-arginine on atherogenesis and its ability to prevent endothelial dysfunction have been studied extensively during the past years. L-arginine is a substance for nitric oxide synthesis which involves in apoptosis. Hypercholesterolemia promotes endothelial dysfunction, and it is hypothesized that L-arginine prevents endothelial dysfunction through endothelial cells apoptosis inhibition. To test this hypothesis, thirty rabbits were assigned into two groups. The control group received 1% cholesterol diet for 4 weeks, and the L-arginine group received same diets plus 3% L-arginine in drinking water.
No significant differences were observed in cholesterol level between two groups, but the nitrite concentration in L-arginine group was significantly higher than other group (control group: 11.8 +/- 1; L-arginine group: 14.7 +/- 0.5 micromol/l); (p < 0.05). The aorta score of fatty streak in control group was 0.875 +/- 0.35, but no fatty streak lesion was detected in L-arginine group (p < 0.05). The number of intimal apoptotic cells/500 cells of aorta in two groups of experiment were statistically different (control group: 39.3 +/- 7.6; L-arginine group: 21.5 +/- 5.3) (p < 0.05).
The inhibition of endothelial cells apoptosis by L-arginine restores endothelial function in a model of hypercholesterolemia.
在过去几年中，已对L-精氨酸对动脉粥样硬化形成的影响及其预防内皮功能障碍的能力进行了广泛研究。L-精氨酸是一种参与细胞凋亡的一氧化氮合成物质。高胆固醇血症会促进内皮功能障碍，据推测，L-精氨酸通过抑制内皮细胞凋亡来预防内皮功能障碍。为验证这一假设，将30只兔子分为两组。对照组给予1%胆固醇饮食4周，L-精氨酸组给予相同饮食并在饮用水中添加3%L-精氨酸。
两组之间的胆固醇水平未观察到显著差异，但L-精氨酸组的亚硝酸盐浓度显著高于另一组（对照组：11.8±1；L-精氨酸组：14.7±0.5微摩尔/升）；（p<0.05）。对照组主动脉脂肪条纹评分是0.875±0.35，但L-精氨酸组未检测到脂肪条纹病变（p<0.05）。两组实验中主动脉内膜凋亡细胞数/500个细胞具有统计学差异（对照组：39.3±7.6；L-精氨酸组：21.5±5.3）（p<0.05）。
在高胆固醇血症模型中，L-精氨酸抑制内皮细胞凋亡可恢复内皮功能。
