Olson G E, Winfrey V P
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Anat Rec. 1991 Jun;230(2):209-17. doi: 10.1002/ar.1092300208.
The distribution of actin in spermatogenic cells and epididymal spermatozoa of the opossum, Monodelphis domestica, was examined by immunofluorescence microscopy to identify its potential function in the major structural events of sperm development. In spermatogenic cells actin was located at the site of initial interaction between the nucleus and acrosome and remained present through subsequent acrosome morphogenesis. Actin was also associated both with the posterior pole of the nucleus, at the site of flagellar attachment, and with the manchette. Thus actin may play a role in establishing the specific associations of spermatid organelles and in the streamlining of the cells' architecture. In epididymal spermatozoa two sites of actin localization are present. The first site is surrounding the connecting piece where it may participate in the characteristic 90 degrees rotation of the head. The second site was a ring of actin surrounding the lateral boundary of the acrosome where it may play a role in the sperm pairing process which also occurs in the epididymis.
通过免疫荧光显微镜检查了家负鼠（Monodelphis domestica）生精细胞和附睾精子中肌动蛋白的分布，以确定其在精子发育主要结构事件中的潜在功能。在生精细胞中，肌动蛋白位于细胞核与顶体最初相互作用的部位，并在随后的顶体形态发生过程中一直存在。肌动蛋白还与细胞核的后极（鞭毛附着部位）以及环行带相关。因此，肌动蛋白可能在建立精子细胞细胞器的特定关联以及细胞结构的流线型化中发挥作用。在附睾精子中，存在两个肌动蛋白定位位点。第一个位点围绕着连接段，可能参与头部特征性的90度旋转。第二个位点是围绕顶体侧边界的肌动蛋白环，可能在附睾中也发生的精子配对过程中发挥作用。
