Veronesi Michael C, Kubek Daniel J, Kubek Michael J
Program in Medical Neuroscience, Indiana University School of Medicine, 635 Barnhill Drive, Room MS5022, Indianapolis, IN 46202, United States.
Epilepsy Res. 2008 Nov;82(1):15-20. doi: 10.1016/j.eplepsyres.2008.06.007. Epub 2008 Jul 31.
Neuroexcitatory effects of isoflurane during or following anesthesia are controversial, particularly in epileptic patients. In contrast, halothane is generally considered to be highly anticonvulsant. Kindling is an animal model of epilepsy suitable for studying the effects of anesthetic agents on the epileptic brain. Fully kindled, Sprague-Dawley rats were either untreated or received a 5 min exposure to isoflurane or halothane 30 min prior to a seizure and compared to seizures in the absence of prior anesthesia. Afterdischarge duration was assessed via electroencephalographs recorded from electrodes implanted in the basolateral amygdala and behavioral seizure stereotypy (stages I-V) was simultaneously recorded and analyzed using digital video for all seizures. Total seizure duration and clonus duration were significantly (P<0.05) increased 30 min after isoflurane but not halothane exposure relative to pre-treatment control. These results are the first to demonstrate that isoflurane exacerbates electrically evoked secondarily generalized seizures in fully kindled animals during recovery. These results also show that the kindling paradigm is useful for evaluating the mechanism of anesthetic agents that may be proconvulsant in epileptic subjects.
异氟烷在麻醉期间或之后的神经兴奋作用存在争议，尤其是在癫痫患者中。相比之下，氟烷通常被认为具有高度抗惊厥作用。点燃是一种适用于研究麻醉剂对癫痫大脑影响的癫痫动物模型。将完全点燃的斯普拉格-道利大鼠分为未处理组，或在癫痫发作前30分钟接受5分钟异氟烷或氟烷暴露，并与未进行前期麻醉时的癫痫发作情况进行比较。通过植入基底外侧杏仁核的电极记录脑电图来评估放电后持续时间，并使用数字视频同时记录和分析所有癫痫发作的行为性癫痫刻板发作（I-V期）。与预处理对照组相比，异氟烷暴露30分钟后，总癫痫发作持续时间和阵挛持续时间显著（P<0.05）增加，但氟烷暴露后未出现这种情况。这些结果首次表明，异氟烷在完全点燃的动物恢复过程中会加剧电诱发的继发性全身性癫痫发作。这些结果还表明，点燃模型有助于评估可能在癫痫患者中具有促惊厥作用的麻醉剂的作用机制。
