Gandia Jorge, Galino Jorge, Amaral Olavo B, Soriano Aroa, Lluís Carme, Franco Rafael, Ciruela Francisco
Departament Patologia i Terapèutica Experimental, Facultat de Medicina, Universitat de Barcelona, Pavelló de Govern, L'Hospitalet de Llobregat, 08907 Barcelona, Spain.
FEBS Lett. 2008 Sep 3;582(20):2979-84. doi: 10.1016/j.febslet.2008.07.045. Epub 2008 Aug 7.
Despite some caveats, G protein-coupled receptor oligomerization is a phenomenon that is becoming largely accepted. Within these oligomers, however, stoichiometry remains to be elucidated. Here, by using bimolecular fluorescence complementation, we visualized adenosine A(2A) receptor homodimers in living cells, showing no apparent difference in the subcellular distribution when compared to the YFP-labelled adenosine A(2A) receptor protomer. Interestingly, the combination of bimolecular fluorescence complementation and bioluminescence resonance energy transfer techniques allowed us to detect the occurrence of adenosine A(2A) receptors oligomers containing more than two protomers. These results provide new insights into the molecular composition of G protein-coupled receptor oligomers.
尽管存在一些限制条件，但G蛋白偶联受体寡聚化是一种已被广泛接受的现象。然而，在这些寡聚体中，化学计量比仍有待阐明。在这里，我们通过使用双分子荧光互补技术，在活细胞中可视化了腺苷A(2A)受体同二聚体，与YFP标记的腺苷A(2A)受体单体相比，其亚细胞分布没有明显差异。有趣的是，双分子荧光互补技术和生物发光共振能量转移技术的结合使我们能够检测到含有两个以上单体的腺苷A(2A)受体寡聚体的存在。这些结果为G蛋白偶联受体寡聚体的分子组成提供了新的见解。
