Zakaria Rahimah, Rajikin Mohd Hamim, Yaacob Nik Soriani, Nor Norazmi Mohd
School of Medical Sciences, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia.
Acta Histochem. 2009;111(1):52-60. doi: 10.1016/j.acthis.2008.04.002. Epub 2008 Aug 3.
The aim of the present study was to analyze the immunolocalization of insulin-like growth factor (IGF)-1 and IGF-2 and their receptors in the oviduct and uterus of control and diabetic mice. Sexually mature female ICR mice aged 6-8 weeks were rendered diabetic by streptozotocin (200 mg/kg, administered intraperitoneally). Oviductal and uterine tissues were obtained from the superovulated control and diabetic mice at 48, 72 and 96 h post-human chorionic gonadotropin (hCG) treatment. Localization of IGF-1, IGF-2, IGF-1R and IGF-2R was determined by immunohistochemistry and a semi-quantitative scoring of immunolabelling was performed using a standardized 5-point system. The immunohistochemical scorings for both IGF-1 and IGF-1R were significantly decreased in the oviducts of diabetic mice at 96 h post-hCG treatment. The scores for IGF-2 were significantly increased in the oviducts of diabetic mice at 48 and 72 h post-hCG treatment, and for IGF-2R at 72 h post-hCG treatment. However, there was no significant difference in the scores of IGFs and their receptors in the uterus of control and diabetic mice. In conclusion, the oviductal immunolabelling for IGFs and their receptors was significantly altered by maternal diabetes, which may be of importance in the pathogenesis of preimplantation diabetic embryopathy.
本研究的目的是分析胰岛素样生长因子（IGF）-1和IGF-2及其受体在对照小鼠和糖尿病小鼠输卵管及子宫中的免疫定位。6-8周龄性成熟的雌性ICR小鼠通过腹腔注射链脲佐菌素（200mg/kg）诱导糖尿病。在人绒毛膜促性腺激素（hCG）处理后48、72和96小时，从超排卵的对照小鼠和糖尿病小鼠获取输卵管和子宫组织。通过免疫组织化学确定IGF-1、IGF-2、IGF-1R和IGF-2R的定位，并使用标准化的5分制对免疫标记进行半定量评分。在hCG处理后96小时，糖尿病小鼠输卵管中IGF-1和IGF-1R的免疫组织化学评分显著降低。在hCG处理后48和72小时，糖尿病小鼠输卵管中IGF-2的评分显著升高，在hCG处理后72小时，IGF-2R的评分显著升高。然而，对照小鼠和糖尿病小鼠子宫中IGF及其受体的评分没有显著差异。总之，母体糖尿病显著改变了输卵管中IGF及其受体的免疫标记，这可能在植入前糖尿病胚胎病的发病机制中具有重要意义。
