碳离子辐照CHO-K1细胞后的细胞存活及染色体畸变

Cell survival and chromosomal aberrations in CHO-K1 cells irradiated by carbon ions.

作者信息

Czub J, Banaś D, Błaszczyk A, Braziewicz J, Buraczewska I, Choiński J, Górak U, Jaskóła M, Korman A, Lankoff A, Lisowska H, Łukaszek A, Szefliński Z, Wójcik A

机构信息

Institute of Physics, Swietokrzyska Academy, ul. Swietokrzyska 15, 25-406 Kielce, Poland.

出版信息

Appl Radiat Isot. 2009 Mar;67(3):447-53. doi: 10.1016/j.apradiso.2008.06.016. Epub 2008 Jun 22.

DOI:10.1016/j.apradiso.2008.06.016

PMID:18676154

Abstract

Chinese hamster ovary CHO-K1 cells were exposed to high LET (12)C-beam (LET: 830 keV/microm) in the dose range of 0-6 Gy and to (60)Co irradiation and the RBE value was obtained. Effects of (12)C-beam exposure on cell survival and chromosomal aberrations were calculated. The chromosomal aberration data were fitted with linear equation. The distribution of aberration in cells was examined with a standard u-test and used to evaluate the data according to Poisson probabilities. The variance to the mean ratio sigma(2)/Y and the dispersion index (u) were determined. Overdispersion was significant (p<0.05) when the value of u exceeded 1.96.

摘要

将中国仓鼠卵巢CHO-K1细胞暴露于剂量范围为0 - 6 Gy的高传能线密度（LET）碳离子束（LET：830 keV/μm）以及钴-60辐射下，获得相对生物学效应（RBE）值。计算了碳离子束照射对细胞存活和染色体畸变的影响。染色体畸变数据采用线性方程拟合。用标准u检验检查细胞中畸变的分布，并根据泊松概率对数据进行评估。确定方差与均值之比σ²/Y和离散指数（u）。当u值超过1.96时，过离散显著（p<0.05）。