从印度不同农业生态气候区域的大豆（Glycine max L.）中分离出的本地慢生根瘤菌的遗传多样性。
Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of India.
作者信息
Appunu Chinnaswamy, N'Zoue Angèle, Laguerre Gisèle
机构信息
Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
出版信息
Appl Environ Microbiol. 2008 Oct;74(19):5991-6. doi: 10.1128/AEM.01320-08. Epub 2008 Aug 1.
Abstract
Fifty isolates from root nodules of soybean plants sampled in five agricultural-ecological-climatic regions of India were analyzed by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene, the intergenic spacer region between the 16S and 23S rRNA genes (IGS), and the nifH and nodC genes. Eight haplotypes assigned to the Bradyrhizobium genus were identified, and the genetic diversity was conserved across regions. Sequence analyses of the IGS and the dnaK, glnII, recA, and nifH genes revealed three groups. One of them (26% of isolates) was assigned to Bradyrhizobium liaoningense. A second group (36% of isolates) was identified as B. yuanmingense but likely forms a new biovar able to nodulate soybean plants. The third lineage (38% of isolates) was different from all described Bradyrhizobium species but showed the same symbiotic genotype as B. liaoningense and B. japonicum bv. glycinearum.
摘要
对从印度五个农业生态气候区采集的大豆植株根瘤中分离出的50个菌株，通过对16S rRNA基因、16S和23S rRNA基因之间的基因间隔区（IGS）以及nifH和nodC基因进行PCR-限制性片段长度多态性分析。鉴定出了8种属于慢生根瘤菌属的单倍型，且各区域间的遗传多样性得以保留。对IGS以及dnaK、glnII、recA和nifH基因的序列分析揭示了三个组。其中一组（占分离菌株的26%）被归为辽宁慢生根瘤菌。第二组（占分离菌株的36%）被鉴定为圆明慢生根瘤菌，但可能形成了一个能够与大豆植株结瘤的新生物变种。第三谱系（占分离菌株的38%）与所有已描述的慢生根瘤菌物种不同，但与辽宁慢生根瘤菌和日本慢生根瘤菌大豆生物变种具有相同的共生基因型。
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