与玉米油相比，不同酚类含量的橄榄油对1,2 - 二甲基肼诱导的大鼠结肠癌发生无影响。

No effects of olive oils with different phenolic content compared to corn oil on 1,2-dimethylhydrazine-induced colon carcinogenesis in rats.

作者信息

Femia Angelo Pietro, Dolara Piero, Servili Maurizio, Esposto Sonia, Taticchi Agnese, Urbani Stefania, Giannini Augusto, Salvadori Maddalena, Caderni Giovanna

机构信息

Dept of Pharmacology, University of Florence, Florence, Italy.

出版信息

Eur J Nutr. 2008 Sep;47(6):329-34. doi: 10.1007/s00394-008-0731-x. Epub 2008 Aug 1.

DOI:10.1007/s00394-008-0731-x

PMID:18677543

Abstract

BACKGROUND

Some epidemiological and experimental studies suggest that olive oil, despite its elevated caloric content, may have protective activity against colon cancer, partially due to its phenolic content. However, little experimental evidence exists to support this claim in vivo.

AIM OF THE STUDY

To test the effect of olive oils with different phenolic content in a well-characterized model of colon carcinogenesis, comparing them with corn oil (CO).

METHODS

F344 rats were fed AIN-76 based diets for the entire experimental period; the diets contained 23% (w/w) of lipids from three different sources: extra-virgin olive oil rich in phenolic compounds (EV), rectified olive oil (ROO) with the same fatty acid composition but devoid of phenolic compounds and CO as a control diet. One week later, rats were induced with 1,2-dimethylhydrazine (DMH) (150 mg/kg b.w. x 2 times) to measure preneoplastic lesions (aberrant crypt foci (ACF) and mucin depleted foci (MDF)) and tumours in the intestine.

RESULTS

Thirteen weeks after DMH, the numbers of ACF and MDF were similar in the different groups (ACF/colon were 344.9 +/- 27.0, 288.6 +/- 28.5 and 289.8 +/- 21.4 in CO, EV and ROO groups, respectively, means +/- SE; MDF/colon were 8.83 +/- 1.2, 8.41 +/- 1.5 and 8.75 +/- 1.6 in CO, EV and ROO groups, respectively, means +/- SE). Thirty-two weeks after DMH, the incidence of tumours (rats with tumours/rats in the group) did not differ among the different groups (20/21, 18/19 and 20/20 in the CO, EV, and ROO groups, respectively). Similarly, the number of tumours/ rat in the colorectum (both adenomas and cancers) was not different in the three different groups (2.33 +/- 0.26, 2.42 +/- 0.41 and 2.25 +/- 0.40 in CO, EV and ROO groups, respectively, means +/- SE).

CONCLUSIONS

Olive oil, irrespective of its phenolic content, does not affect DMH-induced colon carcinogenesis in F344 rats compared with CO.

摘要

背景

一些流行病学和实验研究表明，橄榄油尽管热量含量较高，但可能对结肠癌具有保护作用，部分原因是其酚类成分。然而，体内实验证据很少能支持这一说法。

研究目的

在一个特征明确的结肠癌发生模型中，测试不同酚类含量的橄榄油的效果，并将它们与玉米油（CO）进行比较。

方法

在整个实验期间，给F344大鼠喂食基于AIN - 76的饮食；这些饮食含有23%（w/w）来自三种不同来源的脂质：富含酚类化合物的特级初榨橄榄油（EV）、具有相同脂肪酸组成但不含酚类化合物的精炼橄榄油（ROO）以及作为对照饮食的CO。一周后，用1,2 - 二甲基肼（DMH）（150 mg/kg体重×2次）诱导大鼠，以测量肠道中的癌前病变（异常隐窝灶（ACF）和黏液缺失灶（MDF））以及肿瘤。

结果

DMH处理13周后，不同组的ACF和MDF数量相似（CO组、EV组和ROO组的ACF/结肠分别为344.9±27.0、288.6±28.5和289.8±21.4，均值±标准误；CO组、EV组和ROO组的MDF/结肠分别为8.83±1.2、8.41±1.5和8.75±1.6，均值±标准误）。DMH处理32周后，不同组之间肿瘤发生率（有肿瘤的大鼠/组内大鼠）没有差异（CO组、EV组和ROO组分别为20/21、18/19和20/20）。同样，三种不同组中结肠直肠（腺瘤和癌）的肿瘤/大鼠数量也没有差异（CO组、EV组和ROO组分别为2.33±0.26、2.42±0.41和2.25±0.40，均值±标准误）。