Sprick U, Sprick C
C. und O. Vogt-Institute of Brain Research, University of Düsseldorf, Faculty of Medicine, F.R.G.
Behav Brain Res. 1991 May 15;43(2):175-83. doi: 10.1016/s0166-4328(05)80068-3.
After bilateral neurotoxic hippocampectomy adult rats received bilateral fetal suspension grafts at the lesion sites. Functional efficacy of fetal grafted tissue was substantially improved by subcutaneous administration of carbachol at a dose of 0.01 mg/kg s.c. The drug caused an almost complete restitution of performance in grafted animals in a Morris water-maze test. The progress of behavioral recovery was studied over 3 months postoperatively. Grafts of fetal hippocampal neurons alone improved lesion-induced spatial learning deficits to a limited degree. These results suggest that peripheral administration of carbachol may be of use in the treatment of central lesions associated with cognitive impairments, allowing or enhancing the development of graft-induced amelioration of behavioral deficits. The data also support the view that grafts of embryonic neural tissue may produce a local opening of the blood-brain barrier allowing drugs which normally act only peripherally to exert central effects. These central effects are probably limited to the graft site because the rest of the brain has a blood-brain barrier which is still intact.
成年大鼠在双侧海马神经毒性切除术后，于损伤部位接受双侧胎儿悬浮移植。通过皮下注射剂量为0.01mg/kg的卡巴胆碱，胎儿移植组织的功能疗效得到显著改善。该药物使移植动物在莫里斯水迷宫试验中的行为表现几乎完全恢复。术后3个月对行为恢复进程进行了研究。单独移植胎儿海马神经元仅在有限程度上改善了损伤诱导的空间学习缺陷。这些结果表明，外周给予卡巴胆碱可能有助于治疗与认知障碍相关的中枢损伤，允许或增强移植诱导的行为缺陷改善。数据还支持这样一种观点，即胚胎神经组织移植可能导致血脑屏障局部开放，使通常仅在外周起作用的药物发挥中枢作用。这些中枢作用可能仅限于移植部位，因为大脑其余部分的血脑屏障仍然完好。
