Cirioni Oscar, Ghiselli Roberto, Orlando Fiorenza, Silvestri Carmela, De Luca Stefania, Salzano Anna Maria, Mocchegiani Federico, Saba Vittorio, Scalise Giorgio, Scaloni Andrea, Giacometti Andrea
Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy.
Crit Care Med. 2008 Sep;36(9):2629-33. doi: 10.1097/CCM.0b013e318184430d.
To investigate the efficacy of distinctin in a neutropenic mouse model of staphylococcal sepsis.
Prospective, randomized, and controlled animal study.
Research laboratory in a University Hospital.
BALB/c male mice.
Mice were rendered neutropenic by injecting cyclophosphamide (200 mg/kg of body weight/day) on days -4 and -2 preinfection. Infection was induced at time 0 by intraperitoneal injection of 1 x 10(9) colony forming units of the staphylococcal strain. For each model, all animals were randomized to receive intravenous isotonic sodium chloride solution, 1 mg/kg distinctin, and 10 mg/kg imipenem, 10 mg/kg vancomycin, 10 mg/kg teicoplanin or 10 mg/kg linezolid alone, or combined with 1 mg/kg distinctin.
Lethality, bacterial growth in blood and peritoneum, spleen, liver, and mesenteric lymph nodes.
All combined regimen showed lower lethality rates than singly treated-groups. Distinctin plus vancomycin or teicoplanin exerted the lowest lethality rate. All regimens were significantly superior to controls at reducing blood, spleen, peritoneum, liver and mesenteric lymph node complex bacterial burdens, whereas all combined treated groups were higher effective than singly treated groups.
Our data indicate that distinctin alone or combined with other antibiotics may be useful in treating severe staphylococcal infections.
在中性粒细胞减少的葡萄球菌败血症小鼠模型中研究Distinctin的疗效。
前瞻性、随机对照动物研究。
大学医院的研究实验室。
BALB/c雄性小鼠。
在感染前第-4天和第-2天通过注射环磷酰胺（200mg/kg体重/天）使小鼠中性粒细胞减少。在时间0通过腹腔注射1×10⁹个葡萄球菌菌株的菌落形成单位诱导感染。对于每个模型，所有动物被随机分组，分别接受静脉注射等渗氯化钠溶液、1mg/kg的Distinctin、10mg/kg的亚胺培南、10mg/kg的万古霉素、10mg/kg的替考拉宁或10mg/kg的利奈唑胺单独治疗，或与1mg/kg的Distinctin联合治疗。
死亡率、血液和腹膜、脾脏、肝脏及肠系膜淋巴结中的细菌生长情况。
所有联合治疗方案的死亡率均低于单药治疗组。Distinctin联合万古霉素或替考拉宁的死亡率最低。所有治疗方案在降低血液、脾脏、腹膜、肝脏和肠系膜淋巴结的复合细菌负荷方面均显著优于对照组，而所有联合治疗组比单药治疗组更有效。
我们的数据表明，Distinctin单独使用或与其他抗生素联合使用可能对治疗严重葡萄球菌感染有用。
