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使用特定的DNA探针检测野外疫情中的鸡毒支原体。

The use of a specific dna probe for detection of Mycoplasma gallisepticum in field outbreaks.

作者信息

Levisohn S, Hyman H, Perelman D, Razin S

机构信息

Kimron Veterinary Institute, Beit Dagan, Israel.

出版信息

Avian Pathol. 1989 Jul;18(3):535-41. doi: 10.1080/03079458908418625.

DOI:10.1080/03079458908418625
PMID:18679883
Abstract

A specific DNA probe (pMG4) was used to diagnose M. gallisepticum (MG) infection in field outbreaks in chicken and turkey breeder flocks in Israel. Dot-blot hybridisation of tracheal swab suspensions with the probe enabled positive identification of MG as early as 4 days after sampling, even in flocks at an early stage of infection when no other specific indications of infection were available.

摘要

一种特异性DNA探针(pMG4)被用于诊断以色列鸡和火鸡种鸡群现场疫情中的鸡毒支原体(MG)感染。用该探针与气管拭子悬液进行斑点杂交,即使在感染早期且无其他感染特异性指征的鸡群中,最早在采样后4天就能阳性鉴定出MG。

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