使用特定的DNA探针检测野外疫情中的鸡毒支原体。
The use of a specific dna probe for detection of Mycoplasma gallisepticum in field outbreaks.
作者信息
Levisohn S, Hyman H, Perelman D, Razin S
机构信息
Kimron Veterinary Institute, Beit Dagan, Israel.
出版信息
Avian Pathol. 1989 Jul;18(3):535-41. doi: 10.1080/03079458908418625.
PMID:18679883
Abstract
A specific DNA probe (pMG4) was used to diagnose M. gallisepticum (MG) infection in field outbreaks in chicken and turkey breeder flocks in Israel. Dot-blot hybridisation of tracheal swab suspensions with the probe enabled positive identification of MG as early as 4 days after sampling, even in flocks at an early stage of infection when no other specific indications of infection were available.
摘要
一种特异性DNA探针（pMG4）被用于诊断以色列鸡和火鸡种鸡群现场疫情中的鸡毒支原体（MG）感染。用该探针与气管拭子悬液进行斑点杂交，即使在感染早期且无其他感染特异性指征的鸡群中，最早在采样后4天就能阳性鉴定出MG。
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