Debiec-Rychter M, Azuma M, Zukowski K, Oyasu R, Wang C Y
Department of Chemical Carcinogenesis, Michigan Cancer Foundation, Detroit 48201.
Genes Chromosomes Cancer. 1991 May;3(3):221-30. doi: 10.1002/gcc.2870030309.
The correlation between chromosomal changes and tumorigenic potential of rat bladder epithelial cells was investigated. Seven cell lines were established from neoplastic urothelial cells derived from heterotopically transplanted rat bladders treated with topical application of one of two carcinogens, N-methyl-N-nitrosourea (MNU) or N-hydroxy-N-glucuronosyl-2-aminofluorene. Loss of the short arm of chromosome 3 was demonstrated in three of four lines tumorigenic in the nude mouse, but in none of three nontumorigenic lines. One of the three nontumorigenic cell lines (D44c) was treated further in vitro with MNU. All six tumorigenic, but none of the four nontumorigenic, morphologically altered cell lines derived from D44c demonstrated loss of the short arm of chromosome 3. These results suggest that chromosome 3 alterations may be associated with the tumorigenicity of carcinogen-induced rat bladder epithelial cells.
研究了大鼠膀胱上皮细胞染色体变化与致瘤潜能之间的相关性。从用两种致癌物之一N-甲基-N-亚硝基脲（MNU）或N-羟基-N-葡糖醛酸基-2-氨基芴局部处理的异位移植大鼠膀胱来源的肿瘤性尿路上皮细胞中建立了7个细胞系。在裸鼠中具有致瘤性的4个细胞系中的3个显示出3号染色体短臂缺失，但3个无致瘤性的细胞系中均未出现。对3个无致瘤性细胞系之一（D44c）在体外进一步用MNU处理。从D44c衍生的所有6个具有致瘤性但4个无致瘤性的形态改变细胞系中，均显示出3号染色体短臂缺失。这些结果表明，3号染色体改变可能与致癌物诱导的大鼠膀胱上皮细胞的致瘤性有关。
