Wang Dali, Sun Guangfeng, Wei Zairong, Cui Hongwang, Li Li
Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi Guizhou, 563003, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008 Jul;22(7):842-5.
To explore the effect of burying testis in inguinal pocket on spermatogenesis.
Sixty New Zealand rabbits of 6-8 months old included 36 males and 24 females, weighing 2.5-2.7 kg. The male rabbits were randomly divided into the experimental group (n=18) and the control group (n=18). The model of repairing skin defect of scrotum were established by burying testes in inguinal region subcutaneously in the experimental group. The rabbits were not treated in the control group. The sperms were collected and the surface temperature of testis was measured in both groups after 8 weeks. Testes biopsies were harvested from 6 rabbits of 2 groups randomly respectively. The apoptosis of spermatogenic cells was detected with TUNEL. The other 12 male rabbits in two groups were fed respectively with female rabbits to observe the fertility.
The semen density and the spermid activity ratio were (237.3 +/- 39.7) x 10(9)/L and 76.9% +/- 3.8% in the control group, and were (4.7 +/- 2.7) x 10(9)/L and 0 in the experimental group respectively; showing statistically significant difference between two groups (P< 0.05). The average superficial temperature of testes was (38.02 +/- 0.36) degrees C in the experimental group and (36.15 +/- 0.64) degrees C in the control group (P < 0.05). TUNEL results showed: The spermatogenic epithelium became thin and obvious apoptotic spermatogenic cells were found in experimental group; the spermatogenic epithelium was normal and few apoptotic spermatogenic cells were found in the control group. The apoptotic index (A1) was 89.69% +/- 3.76% in the experimental group and 7.73% +/- 4.95% in the control group (P < 0.05). The Pairing results showed that the female rabbits pairing with male rabbits of the experimental group were all not pregnant, and those of the control group were all pregnant (P < 0.05).
As the same as the scrotum was reconstructed with skin flaps, it will induce the rabbit infertility that the testes were buried in inguinal region subcutaneously to repair defect of scrotum skin. The main reason is the excessive apoptosis of spermatogenic cell by the high testes environmental temperature.
探讨将睾丸埋置于腹股沟袋对精子发生的影响。
60只6 - 8月龄的新西兰兔，其中雄性36只，雌性24只，体重2.5 - 2.7 kg。将雄性兔随机分为实验组（n = 18）和对照组（n = 18）。实验组通过将睾丸皮下埋置于腹股沟区建立阴囊皮肤缺损修复模型，对照组未作处理。8周后收集两组兔的精液并测量睾丸表面温度。分别从两组中随机选取6只兔进行睾丸活检，采用TUNEL法检测生精细胞凋亡情况。两组另外12只雄性兔分别与雌性兔交配观察生育情况。
对照组精液密度和精子活动率分别为（237.3±39.7）×10⁹/L和76.9%±3.8%，实验组分别为（4.7±2.7）×10⁹/L和0，两组比较差异有统计学意义（P < 0.05）。实验组睾丸平均表面温度为（38.02±0.36）℃，对照组为（36.15±0.64）℃（P < 0.05）。TUNEL结果显示：实验组生精上皮变薄，可见明显凋亡的生精细胞；对照组生精上皮正常，凋亡的生精细胞少见。实验组凋亡指数（AI）为89.69%±3.76%，对照组为7.73%±4.95%（P < 0.05）。交配结果显示，与实验组雄性兔交配的雌性兔均未怀孕，与对照组雄性兔交配的雌性兔均怀孕（P < 0.05）。
与采用皮瓣重建阴囊一样，将睾丸皮下埋置于腹股沟区修复阴囊皮肤缺损会导致兔不育。主要原因是睾丸环境温度升高导致生精细胞过度凋亡。
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