Cui Hong-Wang, Wang Da-Li, Sun Guang-Feng, Wei Zai-Rong
Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, China.
Zhonghua Nan Ke Xue. 2011 Sep;17(9):785-9.
To observe the apoptosis of spermatogenic cells and the expressions of Bcl-2 and Bax proteins after burying the testis in the inguinal pocket, and to investigate their relationship.
We randomly divided 36 healthy male New Zealand white rabbits into an experimental group (n = 18) and a control group (n = 18). Models were established by burying testes in the inguinal pocket in the experimental group, while the controls were left untreated. At the end of the 8th week after surgery, 6 animals were randomly taken from each group for measurement of the testis surface temperature and testicular biopsy. The apoptosis of spermatogenic cells in the testis tissues was detected by TUNEL assay, and the expressions of Bcl-2 and Bax proteins determined by immunohistochemistry and imaging analysis.
At 8 weeks after burying the testis in the inguinal pocket, the testicular surface temperature was significantly higher in the experimental group than in the control ([ 38.02 +/- 0.36] degrees C vs [36.15 +/- 0.64 ] degrees C, P < 0.05), and so was the apoptosis index (AI) of spermatogenic cells ([89.69 +/- 3.76] % vs [7.73 +/- 4.95 ] %, P < 0.05). The expression of the Bax protein in the testis was significantly increased, while that of the Bcl-2 protein remarkably decreased in the experimental group as compared with the control group (P < 0.05). The apoptotic cells were mostly primary spermatocytes and round spermatids.
Elevated local temperature of the testis buried in the inguinal pocket increases the apoptosis of spermatogenic cells, and the spermatogenic cell apoptosis is highly correlated with the decreased expression of Bcl-2 and increased expression of Bax. The changes in the expressions of Bcl-2 and Bax proteins were a main mechanism behind the temperature elevation-induced apoptosis of spermatogenic cells.
观察将睾丸埋置于腹股沟袋后生精细胞的凋亡情况以及Bcl-2和Bax蛋白的表达，并探讨它们之间的关系。
将36只健康雄性新西兰白兔随机分为实验组（n = 18）和对照组（n = 18）。实验组通过将睾丸埋置于腹股沟袋建立模型，而对照组不做处理。术后第8周结束时，每组随机取6只动物测量睾丸表面温度并进行睾丸活检。采用TUNEL法检测睾丸组织中生精细胞的凋亡情况，通过免疫组化和图像分析测定Bcl-2和Bax蛋白的表达。
将睾丸埋置于腹股沟袋8周后，实验组睾丸表面温度显著高于对照组（[38.02±0.36]℃对[36.15±0.64]℃，P < 0.05），生精细胞凋亡指数（AI）也显著高于对照组（[89.69±3.76]%对[7.73±4.95]%，P < 0.05）。与对照组相比，实验组睾丸中Bax蛋白表达显著增加，而Bcl-2蛋白表达显著降低（P < 0.05）。凋亡细胞主要是初级精母细胞和圆形精子细胞。
埋置于腹股沟袋的睾丸局部温度升高会增加生精细胞的凋亡，且生精细胞凋亡与Bcl-2表达降低和Bax表达增加高度相关。Bcl-2和Bax蛋白表达的变化是温度升高诱导生精细胞凋亡的主要机制。
