大鼠肩胛间棕色脂肪组织分离细胞中的L-丙氨酸转运

L-alanine transport in isolated cells of interscapular brown adipose tissue in rat.

作者信息

Rodríguez-Martín A, Remesar X

机构信息

Departament de Boquímica i Fisiologia, Universitat de Barcelona, Spain.

出版信息

Biosci Rep. 1991 Apr;11(2):65-71. doi: 10.1007/BF01119193.

DOI:10.1007/BF01119193

PMID:1868173

Abstract

The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd = 0.55 min-1) and a saturable Na(+)-dependent transport (KM = 0.87 mM and Vmax = 155 nmol/min/10(6) cells). The saturable component can be subdivided into MeAIB-sensitive (KM = 1.63 mM and Vmax = 162 nmol/min/10(6) cells) and MeAIB-insensitive (KM = 3.2 mM and Vmax = 39.5 nmol/min/10(6) cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several tissues.

摘要

已确定肩胛间棕色脂肪组织分离细胞中L-丙氨酸的摄取模式。摄取可分为扩散成分（Kd = 0.55分钟-1）和可饱和的Na(+)依赖性转运（KM = 0.87 mM，Vmax = 155 nmol/分钟/10(6)个细胞）。可饱和成分可细分为对甲基胺异丁基丙氨酸（MeAIB）敏感的（KM = 1.63 mM，Vmax = 162 nmol/分钟/10(6)个细胞）和对MeAIB不敏感的（KM = 3.2 mM，Vmax = 39.5 nmol/分钟/10(6)个细胞）。这种动力学模式可能表明存在类似于几种组织中常见的丙氨酸摄取转运系统的转运系统。