使用小型自动化液氮系统冷冻保存的脐带血祖细胞的质量评估。

Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system.

作者信息

Miura Junko, Minegishi Masayoshi, Itoh Tsuneo, Kitaura Tamie, Fukawa Narumi, Takahashi Hiroyuki, Suzuki Akira, Kudo Yoshinori, Narita Ayuko, Sato Yuko, Suzuki Masakuni, Wada Yuichi, Takeyama Yoichi, Watanabe Takanori, Tsuchiya Shigeru

机构信息

Division of Blood Transfusion, Tohoku University Hospital, 1-1 Seiryomachi, Aoba-ku, Sendai, Miyagi Prefecture 980-8574, Japan.

出版信息

Cryobiology. 2008 Oct;57(2):178-81. doi: 10.1016/j.cryobiol.2008.07.004. Epub 2008 Jul 19.

DOI:10.1016/j.cryobiol.2008.07.004

PMID:18682250

Abstract

The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), and total CFU were analyzed. Twenty UCB units cryopreserved using the automated system and stored for a median of 34 days were analyzed. Mean CD34+ cell viabilities before freezing were 99.8+/-0.5% and after thawing were 99.8+/-0.4% in the large bag compartments and 99.7+/-0.5% in the small compartments. The mean recovery values for total nucleated cells, CD34+ cells, CFU-GM, and total CFU were 94.8+/-16.0%, 99.3+/-18.6%, 103.9+/-20.6%, and 94.3+/-12.5%, respectively in the large compartments, and 95.8+/-25.9%, 106.8+/-23.9%, 101.3+/-23.3%, and 93.8+/-19.2%, respectively in the small compartments. A small-scale automated cryopreservation and storage system did not impair the clonogenic capacity of UCB HPCs. This cryopreservation system could provide cellular products adequate for UCB banking and HPC transplantation.

摘要

对用于脐带血（UCB）单位储存的小型自动化冷冻保存和存储系统（Mini-BioArchive系统）的性能进行了评估。解冻这些单位后，分析了细胞的活力和回收率，以及造血祖细胞（HPC）如CD34+细胞、集落形成单位-粒细胞-巨噬细胞（CFU-GM）和总CFU的回收率。分析了使用自动化系统冷冻保存并储存中位数为34天的20个UCB单位。大袋隔室中冷冻前CD34+细胞的平均活力为99.8±0.5%，解冻后为99.8±0.4%，小隔室中为99.7±0.5%。大隔室中总核细胞、CD34+细胞、CFU-GM和总CFU的平均回收值分别为94.8±16.0%、99.3±18.6%、103.9±20.6%和94.3±12.5%，小隔室中分别为95.8±25.9%、106.8±23.9%、101.3±23.3%和93.8±19.2%。小型自动化冷冻保存和存储系统不会损害UCB HPC的克隆能力。这种冷冻保存系统可以提供适合UCB储存和HPC移植的细胞产品。