Liu Jin-Hui, He Ji, Zhu Fa-Ming, Yan Li-Xing
Blood Center of Zhejiang Province, Hangzhou 310006, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Feb;13(1):143-6.
The study was aimed to establish a standard procedure for human umbilical cord blood bank. The hematopoietic nucleated cells in cord blood were processed by using sedimentation and centrifugation method. After finishing CD34(+) cell counting, hematopoietic progenitor cell assay, microbial culture, infectious disease test and HLA typing, cord blood units were stored in the liquid nitrogen for further application. The results showed that nucleated cells of cord blood were (10.94 +/- 2.74) x 10(8) per unit; recovery rate of nucleated cells was (79.82 +/- 17.76)%. CD34(+) cells in cord blood were counted as (51.62 +/- 30.53) x 10(5) per unit. Eight units of cord blood were thawed after two years of cryopreservation, the recovery rate of nucleated cells, CD34(+) cells and CFU-GM were (91.4 +/- 6.0)%, (84.6 +/- 20.0)% and (85.8 +/- 14.9)% respectively. It is suggested that the methods and procedure reported for processing and cryopreservation of hematopoietic stem/progenitor cells in the human umbilical cord blood is effective.
本研究旨在建立人类脐带血库的标准程序。采用沉降和离心法处理脐带血中的造血有核细胞。完成CD34(+)细胞计数、造血祖细胞测定、微生物培养、传染病检测和HLA分型后，将脐带血单位储存于液氮中以备进一步应用。结果显示，脐带血有核细胞每单位为(10.94±2.74)×10(8)；有核细胞回收率为(79.82±17.76)%。脐带血中CD34(+)细胞每单位计数为(51.62±30.53)×10(5)。8个脐带血单位在冷冻保存两年后解冻，有核细胞、CD34(+)细胞和CFU-GM的回收率分别为(91.4±6.0)%、(84.6±20.0)%和(85.8±14.9)%。提示所报道的人类脐带血造血干/祖细胞处理和冷冻保存方法及程序是有效的。
