Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065, Japan.
Glycoconj J. 2009 Nov;26(8):987-98. doi: 10.1007/s10719-008-9156-2.
Assay methods for representative glycosyltransferases were described, covering those involved in the synthesis of glycosphingolipids, N-glycans, O-glycans, and proteoglycan glycosaminoglycans. In addition, intracellular localization of glycosyltransferase was comprehensively summarized. Lastly, complex formation of glycosyltransferase proteins with related molecules including subunits, chaperones, and enzyme regulators, that have been recently reported was also summarized.
代表性糖基转移酶的检测方法有描述,包括涉及糖脂、N-聚糖、O-聚糖和蛋白聚糖糖胺聚糖合成的酶。此外,还全面总结了糖基转移酶的细胞内定位。最后,还总结了最近报道的糖基转移酶蛋白与相关分子(包括亚基、伴侣蛋白和酶调节剂)的复合物形成。