Del Bue M, Riccò S, Ramoni R, Conti V, Gnudi G, Grolli S
Department of Animal Health, Veterinary Biotechnology, Food Quality and Safety, University of Parma, via del Taglio 8, Parma 43100, Italy.
Vet Res Commun. 2008 Sep;32 Suppl 1:S51-5. doi: 10.1007/s11259-008-9093-3.
Equine mesenchymal stem cells (MSC) are of particular interest both for basic research and for the therapeutic approach to musculoskeletal diseases in the horse. Their multilineage differentiation potential gives them the capability to contribute to the repair of tendon, ligament and bone damage. MSCs are also considered a promising therapeutic aid in allogeneic cell transplantation, since they show low immunogenicity and immunomodulating functions.Adipose tissue-derived adult equine stem cells (AdMSC) can be isolated, expanded in vitro and then inoculated into the damaged tissue, eventually in the presence of a biological scaffold. Here we report our preliminary experience with adipose-derived mesenchymal stem cells in allogeneic cell-therapy of tendonitis in the horse. MSCs, derived from visceral adipose tissue, were grown in the presence of autologous platelet lysate and characterized for their differentiation and growth potential. Expanded AdMSC were inoculated into the damaged tendon after their dispersion in activated platelet-rich plasma (PRP), a biological scaffold that plays an important role in maintaining cells in defect sites and contributes to tissue healing. Fourteen out of sixteen treated horses showed a functional recovery and were able to return to their normal activity.
马间充质干细胞（MSC）在基础研究以及马肌肉骨骼疾病的治疗方法方面都备受关注。它们的多向分化潜能使它们有能力促进肌腱、韧带和骨骼损伤的修复。由于间充质干细胞显示出低免疫原性和免疫调节功能，因此在同种异体细胞移植中也被认为是一种有前景的治疗辅助手段。脂肪组织来源的成年马干细胞（AdMSC）可以被分离、在体外扩增，然后接种到受损组织中，最终在生物支架存在的情况下进行。在此，我们报告我们在马肌腱炎同种异体细胞治疗中使用脂肪来源间充质干细胞的初步经验。源自内脏脂肪组织的间充质干细胞在自体血小板裂解液存在的情况下生长，并对其分化和生长潜能进行表征。将扩增后的AdMSC分散在活化的富血小板血浆（PRP）中后接种到受损肌腱中，PRP是一种生物支架，在将细胞维持在缺损部位并促进组织愈合方面发挥重要作用。16匹接受治疗的马中有14匹显示功能恢复，并能够恢复正常活动。
