Ye Lin-xiu, Yang Mao-ping, Qiu Hong, Guo Kun-quan, Yan Jin-song
Department of Endocrinology, the Affiliated Dongfeng Hospital, Yunyang Medical College, Shiyan, Hubei, 442008 People's Republic of China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Aug;25(4):452-4.
To investigate the association of the polymorphism in manganese superoxide dismutase (Mn-SOD) gene in Chinese type 2 diabetic patients with diabetic retinopathy.
The Ala(-9)Val polymorphism of the Mn-SOD gene was determined by polymerase chain reaction and direct sequencing in 198 normal control subjects and 264 patients with type 2 diabetes mellitus, among them there were 139 non-diabetic retinopathy (NDR) subjects and 125 subjects with diabetic retinopathy (DR).
There was no statistic difference in the frequencies of VV genotype and V allele between the type 2 diabetic group and the control group. However, the frequencies of VV genotype and V allele were significantly higher in the DR group than that in the NDR group (chi-square (2)=5.015, P=0.025; chi(2)=10.253, P=0.001),but there was no statistic difference in the NDR group compared with the control group (P > 0.05). The presence of V allele was shown to be associated with diabetic retinopathy (OR=1.96, 95%CI: 1.29-2.97). Furthermore, the subjects carrying the VV genotype had lower serum Mn-SOD level (P=0.025) and had a tendency of higher total serum SOD activity, but this tendency had no statistic significance.
The Ala(-9)Val polymorphism in the Mn-SOD gene may not be related to the etiology of type 2 diabetes, but it seems to contribute to the development of diabetic retinopathy in Chinese type 2 diabetic patients.
研究中国2型糖尿病患者锰超氧化物歧化酶（Mn-SOD）基因多态性与糖尿病视网膜病变的关系。
采用聚合酶链反应和直接测序法，对198例正常对照者和264例2型糖尿病患者进行Mn-SOD基因Ala（-9）Val多态性检测，其中2型糖尿病患者中139例无糖尿病视网膜病变（NDR），125例有糖尿病视网膜病变（DR）。
2型糖尿病组与对照组之间VV基因型和V等位基因频率无统计学差异。然而，DR组的VV基因型和V等位基因频率显著高于NDR组（χ²(2)=5.015，P=0.025；χ(2)=10.253，P=0.001），但NDR组与对照组相比无统计学差异（P>0.05）。V等位基因的存在与糖尿病视网膜病变相关（OR=1.96，95%CI：1.29-2.97）。此外，携带VV基因型的患者血清Mn-SOD水平较低（P=0.025），总血清SOD活性有升高趋势，但无统计学意义。
Mn-SOD基因Ala（-9）Val多态性可能与2型糖尿病的病因无关，但似乎与中国2型糖尿病患者糖尿病视网膜病变的发生有关。
