Cottenye Nicolas, Teixeira Francisco, Ponche Arnaud, Reiter Günter, Anselme Karine, Meier Wolfgang, Ploux Lydie, Vebert-Nardin Corinne
Institut de Chimie des Surfaces et Interfaces, CNRS UPR 9069, Mulhouse Cedex, France.
Macromol Biosci. 2008 Dec 8;8(12):1161-72. doi: 10.1002/mabi.200800081.
Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide-based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide-modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria.
设计了允许形貌和化学成分独立变化的寡核苷酸模型表面，以研究大肠杆菌的粘附和生物膜生长。通过寡核苷酸的共价结合和基于核苷酸的囊泡的固定来制备表面。在该过程的每个步骤中，通过荧光显微镜、X射线光电子能谱、椭偏仪、原子力显微镜和润湿性研究相结合的方式对其性质进行了确认。然后使用这些表面来研究在静态生物膜生长模式下定量的三种不同大肠杆菌菌株的反应。这项研究提供了令人信服的证据，即寡核苷酸修饰的表面，与本研究中使用的形貌特征无关，可增强卷曲菌毛的表达，而不会增加粘附细菌的数量。
