牛分枝杆菌和结核分枝杆菌H37Rv感染期间小鼠巨噬细胞体外产生γ干扰素、肿瘤坏死因子-α和白细胞介素-6

Ex vivo production of interferon-gamma, tumor necrosis factor-alpha, and interleukin-6 by mouse macrophages during infection with M. bovis and M. tuberculosis H37Rv.

作者信息

Chernousova L N, Smirnova T G, Afanasieva E G, Karpov V L, Timofeev A V

机构信息

Central Research Institute of Tuberculosis of the Russian Academy of Medical Sciences, Moscow.

出版信息

Bull Exp Biol Med. 2007 Nov;144(5):709-12. doi: 10.1007/s10517-007-0412-4.

DOI:10.1007/s10517-007-0412-4

PMID:18683503

Abstract

Ex vivo production of IFN-gamma, TNF-alpha, and IL-6 by mouse peritoneal macrophages was studied during successive infection with the vaccine strain M. bovis BCG and virulent strain M. tuberculosis H37Rv. The increase in the concentrations of TNF-alpha and IL-6 did not depend on the sequence of macrophage infection with the vaccine or virulent strain, but was related to the presence of the vaccine strain M. bovis BCG in the medium. IFN-gamma production depended on infection of macrophages with the virulent strain M. tuberculosis H37Rv. The concentration of IFN-gamma was maximum during primary infection with the virulent strain and did not increase after successive infection with the virulent and vaccine strain.

摘要

在小鼠腹腔巨噬细胞先后感染卡介苗菌株牛分枝杆菌（M. bovis BCG）和强毒株结核分枝杆菌（M. tuberculosis H37Rv）的过程中，研究了其体外产生γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的情况。TNF-α和IL-6浓度的增加并不取决于巨噬细胞先感染疫苗株还是强毒株的顺序，而是与培养基中牛分枝杆菌卡介苗菌株的存在有关。IFN-γ的产生取决于巨噬细胞被结核分枝杆菌强毒株H37Rv感染。在初次感染强毒株期间，IFN-γ的浓度最高，在先后感染强毒株和疫苗株后并未增加。