[水通道蛋白-8在妊娠肝内胆汁淤积症大鼠肝细胞中的表达]

[Expression of aquaporin-8 in the hepatocytes of pregnant rats with intrahepatic cholestasis].

作者信息

Li Li-Qiong, Qi Hong-Bo

机构信息

Department of Obstetrics and Gynecology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2008 Feb;43(2):128-31.

PMID:18683753

Abstract

OBJECTIVE

To investigate the alteration on the expression levels of protein and messenger RNA (mRNA) of aquaporin-8 (AQP8) in the hepatocytes of pregnant rats with intrahepatic cholestasis induced by ethinylestradiol.

METHODS

A total number of 20 15-day pregnant rats were randomly divided into two groups: control group, intrahepatic cholestasis of pregnancy (ICP) group. In ICP group, rats were subcutaneously injected with ethinylestradiol (2.5 mg x kg(-1) x d(-1)) for 5 days to make the model of ICP. In control group, rats received subcutaneous injection of appropriate volume of propylene glycol for 5 days. The protein expression and subcellular localization of AQP8 in the hepatocytes of pregnant rats were detected using immunohistochemical methods. The mRNA expression of AQP8 in the hepatocytes was assayed by RT-PCR method.

RESULTS

The model of ICP of pregnant rats was made successfully. Expressions of AQP8 protein and mRNA were detected in two groups. AQP8 protein level in ICP group was 10.8 +/- 2.4, significantly decreased than in control group 17.1 +/- 2.2 (P < 0.05). AQP8 mRNA level in ICP group was 1.07 +/- 0.11, significantly higher than in control group 0.80 +/- 0.11 (P < 0.05).

CONCLUSION

Down-regulated AQP8 protein expression and up-regulated AQP8 mRNA expression in the hepatocytes of pregnant rats with intrahepatic cholestasis may contribute to the pathogenesis of ICP.

摘要

目的

探讨乙炔雌二醇诱导的妊娠肝内胆汁淤积症大鼠肝细胞中水通道蛋白8（AQP8）的蛋白质和信使核糖核酸（mRNA）表达水平的变化。

方法

将20只孕15天的大鼠随机分为两组：对照组、妊娠肝内胆汁淤积症（ICP）组。ICP组大鼠皮下注射乙炔雌二醇（2.5mg·kg⁻¹·d⁻¹），连续5天，以建立ICP模型。对照组大鼠皮下注射等量的丙二醇，连续5天。采用免疫组织化学方法检测妊娠大鼠肝细胞中AQP8的蛋白质表达及亚细胞定位。采用逆转录-聚合酶链反应（RT-PCR）法检测肝细胞中AQP8的mRNA表达。