Leitzel K, Bryce W, Tomita J, Manderino G, Tribby I, Thomason A, Billingsley M, Podczaski E, Harvey H, Bartholomew M
Department of Medicine, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033.
Cancer Res. 1991 Aug 15;51(16):4149-54.
Platelet-derived growth factor (PDGF) is produced by a variety of normal and tumor cells in vitro. We have developed an enzyme-linked immunosorbent assay for the detection of the B-chain of PDGF. This assay can reliably detect 0.1 ng/ml of homodimeric recombinant PDGF B-chain and does not cross-react with recombinant PDGF-AA, epidermal growth factor, basic fibroblast growth factor, or transforming growth factor-beta. Citrated plasma from 72 control individuals had a PDGF B-chain (PDGF-B) level of 0.32 +/- 0.14 ng/ml (mean +/- SD) with a range of 0.10-0.69 ng/ml. The plasma platelet factor 4 (PF4) level was 97 +/- 70 ng/ml, with a range of 34-363 ng/ml. Citrated plasma was obtained from 131 cancer patients, and plasma PDGF-B was elevated in 19 (15%) of the patients. Both PDGF-B and PF4 were elevated in 14 (11%) of these patients, consistent with a platelet source of PDGF-B. In 5 patients (4%), however, PDGF-B was elevated and PF4 was not elevated compared to the control group. This last group of patients may have a tumor-derived source of PDGF-B which could be important in autocrine or paracrine growth stimulation of the tumor cells.
血小板衍生生长因子（PDGF）可由多种正常细胞和肿瘤细胞在体外产生。我们已开发出一种酶联免疫吸附测定法用于检测PDGF的B链。该测定法能够可靠地检测出0.1 ng/ml的同二聚体重组PDGF B链，且与重组PDGF-AA、表皮生长因子、碱性成纤维细胞生长因子或转化生长因子-β无交叉反应。72名对照个体的枸橼酸盐血浆中，PDGF B链（PDGF-B）水平为0.32±0.14 ng/ml（均值±标准差），范围在0.10 - 0.69 ng/ml之间。血浆血小板因子4（PF4）水平为97±70 ng/ml，范围在34 - 363 ng/ml之间。从131名癌症患者中获取了枸橼酸盐血浆，其中19名患者（15%）的血浆PDGF-B升高。在这些患者中，有14名（11%）的PDGF-B和PF4均升高，这与PDGF-B的血小板来源一致。然而，与对照组相比，有5名患者（4%）的PDGF-B升高而PF4未升高。最后这组患者的PDGF-B可能来源于肿瘤，这对于肿瘤细胞的自分泌或旁分泌生长刺激可能具有重要意义。
