气相色谱-质谱法测定大鼠血浆中龙脑油并研究其药代动力学
Determination and pharmacokinetics of ascaridole in rat plasma by gas chromatography-mass spectrometry.
作者信息
Chu Yang, Li Wei, Han Jianping, Mo Hongmei, Li Xinxin, Zhou Shuiping, Zhu Yonghong, Liu Changxiao
机构信息
Tasly R&D Institute, Tianjin Tasly Group Co. Ltd., Tianjin 300410, China.
出版信息
J Pharm Biomed Anal. 2008 Nov 4;48(3):997-1000. doi: 10.1016/j.jpba.2008.06.017. Epub 2008 Jul 3.
A rapid and sensitive method for determination of ascaridole in rat plasma was developed based on gas chromatography-mass spectrometry (GC/MS). The analyte and internal standard (IS), naphthalene, were extracted from plasma with ethyl acetate and then separated by GC on a HP-5MS capillary analytical column (30 m x 0.25 mm, 0.25 microm) and determined by a quadrupole mass spectrometer detector operated under selected ion monitoring mode (SIM). Excellent linearity was found to be from 10 to 1,000 ng/mL with a lower limit of quantitation (LLOQ) of 10 ng/mL. The accuracy was between 85.3% and 114.0%, and the precision was less than 14.5% (intra- and inter-day). The method was successfully applied to investigate the pharmacokinetic study of ascaridole in rats after a single oral dose of 30, 60 and 120 mg/kg, respectively.
基于气相色谱-质谱联用（GC/MS）技术，开发了一种快速、灵敏的测定大鼠血浆中驱蛔脑的方法。分析物和内标物萘用乙酸乙酯从血浆中萃取出来，然后在HP-5MS毛细管分析柱（30 m×0.25 mm，0.25 µm）上通过气相色谱分离，并用在选择离子监测模式（SIM）下运行的四极杆质谱检测器进行测定。线性关系良好，范围为10至1000 ng/mL，定量下限（LLOQ）为10 ng/mL。准确度在85.3%至114.0%之间，精密度小于14.5%（日内和日间）。该方法成功应用于分别单次口服30、60和120 mg/kg驱蛔脑后大鼠的药代动力学研究。
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