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莱茵衣藻中一种既不属于I型也不属于II型的与光系统II相关的叶绿素a/b结合蛋白（CAB）编码cDNA的特性分析。

Characterization of a cDNA encoding a PSII-associated chlorophyll a/b-binding protein (CAB) from Chlamydomonas moewusii fitting into neither type I nor type II.

作者信息

Larouche L, Tremblay C, Simard C, Bellemare G

机构信息

Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada.

出版信息

Curr Genet. 1991 Apr;19(4):285-8. doi: 10.1007/BF00355056.

DOI:10.1007/BF00355056
PMID:1868577
Abstract

A full-length 1010-bp cDNA clone from Chlamydomonas moewusii coding for the precursor of a chlorophyll a/b-binding protein (CAB) was characterized. Northern analysis shows hybridization to a single 1150-base light-stimulated mRNA. Complementary hybrid-selected mRNAs were translated in vitro; SDS-PAGE indicates the synthesis of three polypeptides of 25, 27 and 28 kDa. Comparison of the deduced polypeptide sequence with other published CABs reveals greater similarity with PSII-associated proteins but, as with other algal CABs, our sequence does not meet established criteria for inclusion into either type I or type II, so branching of CABs into two types seems to have occurred after the divergence between algae and land plants.

摘要

对莱茵衣藻中一个编码叶绿素a/b结合蛋白（CAB）前体的1010bp全长cDNA克隆进行了表征。Northern分析显示与单一的1150碱基对光刺激mRNA杂交。互补杂交选择的mRNA在体外进行翻译；SDS-PAGE表明合成了25、27和28kDa的三种多肽。将推导的多肽序列与其他已发表的CAB进行比较，发现与PSII相关蛋白有更高的相似性，但与其他藻类CAB一样，我们的序列不符合纳入I型或II型的既定标准，因此CAB分为两种类型似乎发生在藻类和陆地植物分化之后。

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本文引用的文献

1
The major light-harvesting complex of Photosystem II: aspects of its molecular and cell biology.光合作用系统 II 的主要光捕获复合物：其分子和细胞生物学方面。
Photosynth Res. 1988 Apr;16(1-2):41-63. doi: 10.1007/BF00039485.
2
Cytokinins modulate the expression of genes encoding the protein of the light-harvesting chlorophyll a/b complex.细胞分裂素调节编码捕光叶绿素 a/b 复合物蛋白的基因的表达。
Plant Mol Biol. 1985 May;5(3):155-63. doi: 10.1007/BF00015679.
3
Expression of Light-Harvesting Chlorophyll a/b-Protein Genes Is Phytochrome-Regulated in Etiolated Arabidopsis thaliana Seedlings.
绿藻门丝藻属 Mantoniella squamata 的叶绿素 a/b/c 结合蛋白的多肽序列。
Photosynth Res. 1994 Jun;40(3):269-77. doi: 10.1007/BF00034776.
4
Influence of photoheterotrophy on the expression of chlorophyll a/b-binding proteins in the green alga Pyrobotrys stellata.光照异养对绿藻Pyrobotrys stellata 叶绿素 a/b 结合蛋白表达的影响。
Photosynth Res. 1996 Jul;49(1):49-56. doi: 10.1007/BF00029427.
5
The atypical chlorophyll a/b/c light-harvesting complex of Mantoniella squamata: molecular cloning and sequence analysis.曼氏藻（Mantoniella squamata）的非典型叶绿素a/b/c捕光复合体：分子克隆与序列分析
Mol Gen Genet. 1993 Sep;240(3):403-13. doi: 10.1007/BF00280392.
6
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Curr Genet. 1993 May-Jun;23(5-6):512-7. doi: 10.1007/BF00312644.
7
Cloning and nucleotide sequence of a cDNA encoding a major fucoxanthin-, chlorophyll a/c-containing protein from the chrysophyte Isochrysis galbana: implications for evolution of the cab gene family.来自金藻等鞭金藻的编码主要岩藻黄质 - 叶绿素a/c结合蛋白的cDNA的克隆及核苷酸序列：对cab基因家族进化的启示
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8
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Plant Physiol. 1988 Dec;88(4):1323-31. doi: 10.1104/pp.88.4.1323.
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
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Comparative biochemistry of photosynthetic light-harvesting systems.光合捕光系统的比较生物化学
Annu Rev Biochem. 1983;52:125-57. doi: 10.1146/annurev.bi.52.070183.001013.
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