Kim Sun Tae, Kang Sun Young, Wang Yiming, Kim Sang Gon, Hwang Du Hyeon, Kang Kyu Young
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, Korea.
Proteomics. 2008 Sep;8(17):3577-87. doi: 10.1002/pmic.200800183.
The phytohormones gibberellic acid (GA) and abscisic acid (ABA) play essential and often antagonistic roles in regulating plant growth, development, and stress responses. Using a proteomics-based approach, we examined the role of GA and ABA in the modulation of protein expression levels during seed germination. Rice seeds were treated with GA (200 microM), ABA (10 microM), ABA followed by GA, GA followed by ABA, and water as a control and then incubated for 3 days. The embryo was dissected from germinated seeds, and proteins were subjected to 2-DE. Approximately, 665 total protein spots were resolved in the 2-D gels. Among them, 16 proteins notably modulated by either GA or ABA were identified by MALDI-TOF MS. Northern analyses demonstrated that expression patterns of 13 of these 16 genes were consistent with those of the proteome analysis. Further examination of two proteins, rice isoflavone resuctase (OsIFR) and rice PR10 (OsPR10), using Western blot and immunolocalization, revealed that both are specifically expressed in the embryo but not in the endosperm and are dramatically downregulated by ABA.
植物激素赤霉素（GA）和脱落酸（ABA）在调节植物生长、发育及应激反应中发挥着重要且往往相互拮抗的作用。我们采用基于蛋白质组学的方法，研究了GA和ABA在种子萌发过程中对蛋白质表达水平的调控作用。用GA（200微摩尔）、ABA（10微摩尔）、先ABA后GA、先GA后ABA处理水稻种子，并以水处理作为对照，然后将种子培养3天。从萌发的种子中分离出胚，对蛋白质进行二维电泳（2-DE）分析。二维凝胶中大约分辨出665个总蛋白点。其中，16个受GA或ABA显著调控的蛋白质通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定出来。Northern分析表明，这16个基因中的13个基因的表达模式与蛋白质组分析结果一致。通过蛋白质免疫印迹法（Western blot）和免疫定位进一步检测水稻异黄酮还原酶（OsIFR）和水稻病程相关蛋白10（OsPR10）这两种蛋白质，结果显示它们均在胚中特异性表达，而不在胚乳中表达，并且受ABA显著下调。
