Pawłowski Tomasz Andrzej
Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland.
Proteomics. 2007 Jun;7(13):2246-57. doi: 10.1002/pmic.200600912.
A proteomic approach was used to analyze mechanisms of dormancy breaking in beech (Fagus sylvatica L.) seeds and the participation of abscisic and gibberellic acids (ABA and GA) in this process. After imbibition in water, ABA, or GA3 solutions, beechnuts were subjected to cold stratification, which breaks their dormancy. ABA delayed, whereas GA3 promoted seed dormancy breaking. Proteome maps for water, ABA, and GA3 were established, which displayed 1544 silver-stained spots. A total of 74 spots, showing significant changes in volume, were identified by MS. Of these, 18, 45, and 16 spots were identified as water-, ABA-, and GA3-responsive, respectively (five were regulated by both hormones). The classification of proteins showed that most of the proteins associated with dormancy breaking in water are involved in energy metabolism and protein destination. Most of the ABA-responsive proteins are involved in protein destination, energy metabolism, and development. Most of the GA3-responsive proteins are involved in energy metabolism (many more than for ABA and water) and plant defense. We conclude that the mechanism of seed dormancy breaking involves the proteins of many processes, beginning with hormone signal initiation, through signal transduction, transcription, protein synthesis, energy metabolism, storage materials, and ending with the cell cycle.
采用蛋白质组学方法分析欧洲山毛榉（Fagus sylvatica L.）种子打破休眠的机制以及脱落酸和赤霉素（ABA和GA）在此过程中的参与情况。在水中、ABA或GA3溶液中吸胀后，对欧洲山毛榉坚果进行低温层积处理，以打破其休眠。ABA延迟种子休眠打破，而GA3促进种子休眠打破。建立了水、ABA和GA3处理的蛋白质组图谱，显示出1544个银染斑点。通过质谱鉴定出总共74个斑点的体积有显著变化。其中，分别有18、45和16个斑点被鉴定为对水、ABA和GA3有响应（5个受两种激素调控）。蛋白质分类表明，大多数与在水中打破休眠相关的蛋白质参与能量代谢和蛋白质定位。大多数ABA响应蛋白参与蛋白质定位、能量代谢和发育。大多数GA3响应蛋白参与能量代谢（比ABA和水响应的蛋白多得多）和植物防御。我们得出结论，种子休眠打破机制涉及许多过程的蛋白质，从激素信号起始开始，经过信号转导、转录、蛋白质合成、能量代谢、储存物质，最后到细胞周期。
