[雌激素对青春期大鼠支持细胞及血睾屏障的影响]
[Estrogens affect Sertoli cells and the blood-testis barrier in pubertal rats].
作者信息
Pan Yi-Qing, Guo Qiang-Su, Wang Ru-Yao, Xu Chen
机构信息
Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
出版信息
Zhonghua Nan Ke Xue. 2008 Jul;14(7):590-6.
OBJECTIVE
To investigate the correlation of exogenous estrogens with the expression of FasL in Sertoli cells and the blood-testis barrier during the differentiation and maturation period of Sertoli cells, and to discuss the related factors that influence the blood-testis barrier of pubertal rats.
METHODS
Super-physiological doses of exogenous estrogenic compounds (diethylstilbestrol and estradiol) were administered to pubertal Sprague-Dawley rats in vitro and in vivo, the FasL expression in the Sertoli cells of the rats detected by immunohistochemistry and Western blot, and the changes in the blood-testis barrier observed with the electron microscope.
RESULTS
After the exposure to exogenous estrogens, the FasL expression was markedly up-regulated in the immature Sertoli cells (P < 0.05) as well as in the Sertoli cell membrane and the blood-testis barrier of the epithelium. The tracer lanthanum passed through the blood-testis barrier and reached the whole layer of the epithelium at 18 days.
CONCLUSION
Super-physiological dose of exogenous estrogens can change the expression and distribution of FasL in immature Sertoli cells and affect the structure of the blood-testis barrier.
目的
探讨外源性雌激素与支持细胞分化成熟过程中FasL表达及血睾屏障的相关性，探讨影响青春期大鼠血睾屏障的相关因素。
方法
对青春期Sprague-Dawley大鼠进行体外和体内超生理剂量外源性雌激素化合物（己烯雌酚和雌二醇）给药，采用免疫组织化学和蛋白质印迹法检测大鼠支持细胞中FasL的表达，用电子显微镜观察血睾屏障的变化。
结果
暴露于外源性雌激素后，未成熟支持细胞中FasL表达明显上调（P<0.05），支持细胞膜及上皮血睾屏障中FasL表达也明显上调。示踪剂镧在18天时穿过血睾屏障并到达上皮全层。
结论
超生理剂量外源性雌激素可改变未成熟支持细胞中FasL的表达和分布，影响血睾屏障结构。
Zotero 插件