Uehara Masae, Sano Ayako, Yarita Kyoko, Kamei Katsuhiko, Haketa Makio, Ide Kyoko, Nagai Keiko, Takayama Yoshihiro, Nishimura Kazuko
Department of Clinical Laboratories, Saku Central Hospital, Nagano, Japan.
Nihon Ishinkin Gakkai Zasshi. 2008;49(3):205-9. doi: 10.3314/jjmm.49.205.
Penicillium marneffei was isolated from three blood cultures of a Thai woman with AIDS and then identified as such. The patient, 41 a year-old female from northeast Thailand came to Japan 10 years ago and married a Japanese man. She was reportedly the third patient infected with this fungal species in Japan, and considered to be the first case from whom the causative fungus was successfully cultured, which led to the diagnosis of penicilliosis marneffei. The colony of the isolate, which was cultured on Sabouraud dextrose agar at 25-27 degrees C, was initially white and pannose, gradually turned in color from yellow to yellow-green, and diffused a deep red pigment into the medium. Conidial heads were divergent, and chains of conidia were formed from phialides. Colonies of the isolate, which was cultured on brain-heart infusion agar at 35 degrees C, had a grayish white, membranous yeast-like form with fine plicae and microscopically consisted of short hyphae. Furthermore, 560 bases of the internal transcribed spacer (ITS) region of the ribosomal RNA gene including the 5.8S region (ITS1-5.8S-ITS2) (DDBJ accession number AB298970) were sequenced and allowed an unequivocal species identification.
马尔尼菲青霉菌从一名患有艾滋病的泰国女性的三次血培养物中分离出来，并据此得以鉴定。该患者为一名41岁的泰国东北部女性，10年前来到日本并与一名日本男子结婚。据报道，她是日本第三例感染这种真菌的患者，被认为是第一例成功培养出致病真菌从而确诊为马尔尼菲青霉病的病例。在25 - 27摄氏度的沙氏葡萄糖琼脂上培养的分离株菌落最初为白色且呈绒毛状，颜色逐渐从黄色变为黄绿，并向培养基中扩散出深红色色素。分生孢子头呈发散状，分生孢子链由瓶梗形成。在35摄氏度的脑心浸液琼脂上培养的分离株菌落呈灰白色、膜状酵母样形态，有细微褶皱，显微镜下由短菌丝组成。此外，对核糖体RNA基因的内部转录间隔区（ITS）包括5.8S区域（ITS1 - 5.8S - ITS2）（DDBJ登录号AB298970）的560个碱基进行了测序，从而明确了物种鉴定。
