Tsai Yuh-Shyan, Jou Yeong-Chin, Lee Gia-Fong, Chen Yeong-Chang, Shiau Ai-Li, Tsai Hsin-Tzu, Wu Chao-Liang, Tzai Tzong-Shin
Institute of Clinical Medicine, University Hospital and Douliou Branch, National Cheng Kung University College of Medicine, Tainan, Taiwan.
Urology. 2009 Jan;73(1):188-92. doi: 10.1016/j.urology.2008.05.031. Epub 2008 Aug 9.
To investigate the prothymosin-alpha (PTMA) expression in human bladder cancer using tissue microarrays.
Two tissue microarray slides of 50 bladder tumors and 42 paired normal adjacent tissues were investigated using immunohistochemical staining. The staining distribution was categorized as negative, nuclear, cytoplasmic, and mixed expression. Quantitative immunoreactivity was measured using image analysis, as represented by the integrated optical density for each tissue core.
In 36 of 42 normal adjacent tissues, positive PTMA immunoreactivity could be seen in some nuclei of the normal urothelial cells, but not, or only minimally, in the cytoplasm and underlying submucosal tissues. A statistically significant enhancement of PTMA expression was found in bladder tumors of each grade compared with the normal adjacent tissue (P < .0001 for normal adjacent tissues vs grade 1, 2, or 3 tumors, paired t test). Of 48 transitional cell carcinoma specimens, only 4 (8.3%) were graded as negative and 44 (91.7%) were positive for PTMA expression, including nuclear (n = 8), cytoplasmic (n = 12), and mixed expression (n = 24) patterns. A statistically significant correlation was found between high grade and mixed expression (P = 0.0020, chi(2) test).
Increased PTMA expression was found in human bladder cancers compared with the paired normal adjacent bladder tissue. The distribution of PTMA expression was changed in high-grade tumors. The clinical significance of such an aberrant PTMA expression in bladder cancer is worthy of additional investigation.
使用组织芯片研究人膀胱癌中前胸腺素α（PTMA）的表达情况。
采用免疫组织化学染色法对包含50例膀胱肿瘤及42例配对正常相邻组织的两张组织芯片载玻片进行研究。染色分布分为阴性、核表达、胞质表达及混合表达。使用图像分析测量定量免疫反应性，以每个组织芯的积分光密度表示。
在42例正常相邻组织中的36例中，正常尿路上皮细胞的一些细胞核中可见PTMA免疫反应性阳性，但在细胞质及下方黏膜下组织中未见或仅见微量阳性。与正常相邻组织相比，各分级膀胱肿瘤中PTMA表达均有统计学意义的增强（正常相邻组织与1级、2级或3级肿瘤比较，配对t检验，P <.0001）。在48例移行细胞癌标本中，仅4例（8.3%）PTMA表达分级为阴性，44例（91.7%）为阳性，包括核表达（n = 8）、胞质表达（n = 12）及混合表达（n = 24）模式。高级别与混合表达之间存在统计学意义的相关性（P = 0.0020，χ²检验）。
与配对的正常相邻膀胱组织相比，人膀胱癌中PTMA表达增加。高级别肿瘤中PTMA表达分布发生改变。膀胱癌中这种异常PTMA表达的临床意义值得进一步研究。
