Ram Siya, Vajpayee Poornima, Singh Ram Lakhan, Shanker Rishi
Environmental Microbiology Division, Indian Institute of Toxicology Research (CSIR), Post Box 80, Mahatma Gandhi Marg, Lucknow 226001, UP, India.
Ecotoxicol Environ Saf. 2009 Feb;72(2):490-5. doi: 10.1016/j.ecoenv.2008.06.006. Epub 2008 Aug 9.
The high incidences of waterborne diseases are frequently associated with shiga toxin (STEC) and enterotoxin producing Escherichia coli (ETEC). Therefore, in the present study, surface water samples collected from the river Saryu were analyzed for the presence of multi-antimicrobial resistant ETEC and STEC. Forty-two E. coli isolates were screened for virulence determinants of STEC and ETEC. Eighteen E. coli isolates exhibit both stx1 and stx2 genes (66.6%) or only stx1 (33.3%) gene. eaeA, hlyA, and chuA genes were present in 94.5%, 83.3%, and 55.6% of STEC, respectively. Further, it was observed that 12 isolates exhibit only ST1 gene (25%) or both LT1 and ST1 genes (75%). The resistance to multi-antimicrobials was observed in 100% and 27.7% of ETEC and STEC isolates, respectively. The presence of multi-antimicrobial resistant diarrheagenic E. coli in surface waters of south Asia is an important health concern due to risk of developing waterborne outbreaks.
水源性疾病的高发病率常常与产志贺毒素大肠杆菌（STEC）和产肠毒素大肠杆菌（ETEC）有关。因此，在本研究中，对从萨鲁河采集的地表水样本进行了分析，以检测多重耐药性ETEC和STEC的存在情况。对42株大肠杆菌分离株进行了STEC和ETEC毒力决定因素的筛选。18株大肠杆菌分离株同时呈现stx1和stx2基因（66.6%）或仅呈现stx1基因（33.3%）。eaeA、hlyA和chuA基因分别存在于94.5%、83.3%和55.6%的STEC中。此外，观察到12株分离株仅呈现ST1基因（25%）或同时呈现LT1和ST1基因（75%）。ETEC和STEC分离株中分别有100%和27.7%呈现多重耐药性。由于存在引发水源性疾病暴发的风险，南亚地表水中存在多重耐药性致泻性大肠杆菌是一个重要的健康问题。
