Mercolini Laura, Mandrioli Roberto, Amore Mario, Raggi Maria Augusta
Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Bologna, Bologna, Italy.
J Sep Sci. 2008 Aug;31(14):2619-26. doi: 10.1002/jssc.200800212.
Benzodiazepines (BZDs) are often prescribed to schizophrenic or depressed patients, as a part of polypharmacy regimens. An HPLC method has been developed for the simultaneous determination of 15 BZDs in human plasma. Separation was obtained by using a C8 RP column and a mobile phase composed of 65% aqueous phosphate buffer at pH 3.0 and 35% ACN. The UV detector was set at 220 nm and clomipramine was used as the internal standard. A careful pretreatment procedure of plasma samples was developed, using SPE with C1 cartridges, which gives high extraction yields (> 97%). The LOQs were always lower than 7.6 ng/mL and the LODs always lower than 2.6 ng/mL for all analytes. The method was successfully applied to plasma samples from depressed and schizophrenic patients undergoing polypharmacy with one or more BZDs. Precision data, as well as accuracy results, were satisfactory and no interference from other drugs was found. Hence, the method seems to be suitable for the therapeutic drug monitoring (TDM) of patients undergoing therapy with one or more BZDs.
苯二氮䓬类药物（BZDs）常作为联合用药方案的一部分，用于精神分裂症或抑郁症患者。已开发出一种高效液相色谱法（HPLC），用于同时测定人血浆中的15种BZDs。采用C8反相柱和由pH 3.0的65%磷酸盐水溶液缓冲液和35%乙腈组成的流动相进行分离。紫外检测器设定在220 nm，氯米帕明用作内标。开发了一种使用C1小柱的固相萃取（SPE）血浆样品预处理程序，其提取率很高（>97%）。所有分析物的定量下限（LOQs）始终低于7.6 ng/mL，检测限（LODs）始终低于2.6 ng/mL。该方法已成功应用于接受一种或多种BZDs联合用药的抑郁症和精神分裂症患者的血浆样本。精密度数据以及准确度结果均令人满意，未发现其他药物的干扰。因此，该方法似乎适用于接受一种或多种BZDs治疗患者的治疗药物监测（TDM）。
