Tiyapongpattana Warawut, Wilairat Prapin, Marriott Philip J
Flow Innovation-Research for Science and Technology Laboratories, Department of Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
J Sep Sci. 2008 Aug;31(14):2640-9. doi: 10.1002/jssc.200800234.
In this work the development of a comprehensive 2-D GC flame ionization detection (GC x GC FID) method for biodiesel fuels is reported. This method is used for the analysis of fatty acid methyl esters (FAMEs) in both biodiesel (B100) and biodiesel blend (B5) samples. The separation of FAME was based on component boiling point in the first dimension and polarity in the second dimension by using a BPX5/BP20 column set to provide a measure of 'orthogonality' in the 2-D space. Here the columns are coupled with a cryogenic modulator operating in a novel temperature programmed mode (T(M)) whereby the cryotrap is progressively incremented in temperature as the oven temperature is increased. The final method employs eight cryotrap temperature settings. The developed GC x GC method is able to successfully characterize and identify both B100 and B5 FAME components, which are produced from a variety of vegetable oils, animal fats and waste cooking oils, with high precision. The method is capable of analysing FAME with carbon numbers C4-C24, and is particularly suitable to characterize various types of biodiesel, making it possible to differentiate the origin and type of FAME used in the biodiesel samples.
本文报道了一种用于生物柴油燃料的综合二维气相色谱火焰离子化检测（GC×GC FID）方法的开发。该方法用于分析生物柴油（B100）和生物柴油混合物（B5）样品中的脂肪酸甲酯（FAMEs）。FAME的分离基于第一维中的组分沸点和第二维中的极性，通过使用BPX5/BP20柱组在二维空间中提供“正交性”的度量。此处，柱子与以新型程序升温模式（T(M)）运行的低温调制器相连，随着柱箱温度升高，冷阱温度逐渐升高。最终方法采用八个冷阱温度设置。所开发的GC×GC方法能够成功地高精度表征和鉴定由各种植物油、动物脂肪和废食用油生产的B100和B5 FAME成分。该方法能够分析碳数为C4 - C24的FAME，特别适用于表征各种类型的生物柴油，从而能够区分生物柴油样品中使用的FAME的来源和类型。
