Perez-Basterrechea Marcos, Briones Ruben M, Alvarez-Viejo Maria, Garcia-Perez Eva, Esteban Manuel M, Garcia Veronica, Obaya Alvaro J, Barneo Luis, Meana Alvaro, Otero Jesus
Transplant and Cell Therapy Unit, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain.
Tissue Eng Part A. 2009 Mar;15(3):569-77. doi: 10.1089/ten.tea.2008.0088.
The transplant of pancreatic islets into the liver can restore normal blood glucose levels in patients with type I diabetes. However, long-term results have indicated that the site and method of transplantation still need to be optimized to improve islet engraftment. This study was designed to assess the efficiency of the use of clotted blood plasma containing fibroblasts ("plasma-fibroblast gel") as a scaffold for subcutaneous islet transplantation in diabetic athymic mice. Islets embedded in the plasma-fibroblast gel were able to resolve hyperglycemia in transplanted mice, restoring normoglycemia over a 60-day period and allowing gradual body weight recovery. Glucose clearances were significantly improved when compared to those recorded in diabetic animals and similar to those observed in the control group (free islets transplanted beneath the kidney capsule). Histological evaluation revealed functional islets within a subcutaneous tissue rich in collagen fibers that was well vascularized, with blood vessels observed around and inside the islets. These findings suggest that this approach could be used as an alternative option for the treatment of type I diabetes in human clinical practice.
将胰岛移植到肝脏中可使I型糖尿病患者的血糖水平恢复正常。然而，长期结果表明，移植部位和方法仍需优化以提高胰岛植入率。本研究旨在评估含有成纤维细胞的凝血血浆（“血浆-成纤维细胞凝胶”）作为糖尿病无胸腺小鼠皮下胰岛移植支架的使用效率。嵌入血浆-成纤维细胞凝胶中的胰岛能够解决移植小鼠的高血糖问题，在60天内恢复正常血糖，并使体重逐渐恢复。与糖尿病动物记录的血糖清除率相比，血糖清除率显著提高，且与对照组（将游离胰岛移植到肾包膜下）观察到的血糖清除率相似。组织学评估显示，在富含胶原纤维且血管化良好的皮下组织内存在功能性胰岛，在胰岛周围和内部均观察到血管。这些发现表明，这种方法可作为人类临床实践中治疗I型糖尿病的替代选择。
