Holmbom B, Lindström M, Näslund U, Thornell L E
Department of Anatomy, University of Umeå, Sweden.
Histochemistry. 1991;95(5):441-7. doi: 10.1007/BF00315739.
A method for large specimen cryosectioning is described. Specimens of pig heart ventricles were lightly fixed by microwave irradiation, embedded in 10% gelatin, frozen in hexan chilled with dry ice, and sectioned using an LKB 2250 PMW cryomicrotome. The sections were collected on transparent film and transferred onto glass slides. Standard histological, enzyme- and immuno-histochemical staining techniques were used. The present method allowed cryosectioning with sections of good quality which could be used for enzyme- and immuno-staining. For studies of experimental myocardial infarction, staining for phosphorylase and Periodic Acid Schiff outlined the ischemic area and antibodies against plasma fibronectin and fibrinogen delineated the infarcted myocardium.
本文描述了一种大标本冷冻切片的方法。猪心室标本经微波照射轻度固定，嵌入10%明胶中，在干冰冷冻的己烷中冷冻，然后用LKB 2250 PMW冷冻切片机切片。切片收集在透明薄膜上，然后转移到载玻片上。使用标准的组织学、酶组织化学和免疫组织化学染色技术。本方法能够切出质量良好的切片，可用于酶染色和免疫染色。对于实验性心肌梗死的研究，磷酸化酶染色和过碘酸希夫反应勾勒出缺血区域，抗血浆纤连蛋白和纤维蛋白原抗体则勾勒出梗死心肌。
