对导致温室职业性过敏的甜菜（Beta vulgaris）花粉中的过敏原蛋白进行鉴定。
The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses.
作者信息
Luoto Susanne, Lambert Wietske, Blomqvist Anna, Emanuelsson Cecilia
机构信息
Department of Biochemistry, Lund University, Lund, Sweden.
出版信息
Clin Mol Allergy. 2008 Aug 11;6:7. doi: 10.1186/1476-7961-6-7.
BACKGROUND
During production of sugar beet (Beta vulgaris) seeds in greenhouses, workers frequently develop allergic symptoms. The aim of this study was to identify and characterize possible allergens in sugar beet pollen.
METHODS
Sera from individuals at a local sugar beet seed producing company, having positive SPT and specific IgE to sugar beet pollen extract, were used for immunoblotting. Proteins in sugar beet pollen extracts were separated by 1- and 2-dimensional electrophoresis, and IgE-reactive proteins analyzed by liquid chromatography tandem mass spectrometry.
RESULTS
A 14 kDa protein was identified as an allergen, since IgE-binding was inhibited by the well-characterized allergen Che a 2, profilin, from the related species Chenopodium album. The presence of 17 kDa and 14 kDa protein homologues to both the allergens Che a 1 and Che a 2 were detected in an extract from sugar beet pollen, and partial amino acid sequences were determined, using inclusion lists for tandem mass spectrometry based on homologous sequences.
CONCLUSION
Two occupational allergens were identified in sugar beet pollen showing sequence similarity with Chenopodium allergens. Sequence data were obtained by mass spectrometry (70 and 25%, respectively for Beta v 1 and Beta v 2), and can be used for cloning and recombinant expression of the allergens. As for treatment of Chenopodium pollinosis, immunotherapy with sugar beet pollen extracts may be feasible.
背景
在温室中生产甜菜（Beta vulgaris）种子期间，工人经常出现过敏症状。本研究的目的是鉴定和表征甜菜花粉中可能的过敏原。
方法
从当地一家甜菜种子生产公司的个体中获取血清，这些个体对甜菜花粉提取物的皮肤点刺试验（SPT）和特异性IgE呈阳性，用于免疫印迹分析。甜菜花粉提取物中的蛋白质通过一维和二维电泳进行分离，IgE反应性蛋白通过液相色谱串联质谱分析。
结果
一种14 kDa的蛋白质被鉴定为过敏原，因为其IgE结合被来自相关物种藜（Chenopodium album）的特征明确的过敏原Che a 2（肌动蛋白结合蛋白）所抑制。在甜菜花粉提取物中检测到与过敏原Che a 1和Che a 2具有同源性的17 kDa和14 kDa蛋白质同源物，并基于同源序列使用串联质谱的包含列表确定了部分氨基酸序列。
结论
在甜菜花粉中鉴定出两种职业性过敏原，它们与藜过敏原具有序列相似性。通过质谱获得了序列数据（Beta v 1和Beta v 2分别为70%和25%），可用于过敏原的克隆和重组表达。至于藜花粉症的治疗，用甜菜花粉提取物进行免疫治疗可能是可行的。
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