Barderas Rodrigo, Villalba Mayte, Lombardero Manuel, Rodríguez Rosalía
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
Int Arch Allergy Immunol. 2002 Jan;127(1):47-54. doi: 10.1159/000048168.
Pollinosis to Chenopodium album has been reported, but no data are available on its allergenic proteins.
An allergen from C. album pollen has been isolated by means of gel permeation and reverse-phase high-performance liquid chromatography. Molecular characterization was achieved by concanavalin A reaction, mass spectrometry, Edman degradation and cDNA sequence. Antigenic analyses were performed by immunoblotting, ELISA, and ELISA inhibition, using sera from allergic patients, two Ole e 1-specific monoclonal antibodies and an Ole e 1-specific polyclonal antiserum.
The isolated allergen, Che a 1, is a glycoprotein of molecular mass 17.088 kD and 143 amino acid residues, whose sequence exhibits 27-45% identity with known members of the Ole e 1-like protein family. 77% of sera from patients allergic to chenopod pollen were reactive to Che a 1. No correlation was found between the IgE reactivities to Che a 1 and Ole e 1, the major allergens from olive pollen, and both allergens display low, although detectable, IgE and IgG cross-reactivities.
Che a 1, a relevant allergen from chenopod pollen, is structurally related to the Ole e 1-like protein family, but exhibits significant differences on its polypeptide sequence that could explain its different antigenic behavior and limited cross-reactivity.
已报道对藜草花粉过敏，但关于其致敏蛋白的数据尚无。
通过凝胶渗透和反相高效液相色谱法从藜草花粉中分离出一种过敏原。通过伴刀豆球蛋白A反应、质谱分析、埃德曼降解法和cDNA序列实现分子表征。使用过敏患者的血清、两种油橄榄Ole e 1特异性单克隆抗体和一种Ole e 1特异性多克隆抗血清，通过免疫印迹法、酶联免疫吸附测定法（ELISA）和ELISA抑制法进行抗原分析。
分离出的过敏原Che a 1是一种分子量为17.088 kD、含143个氨基酸残基的糖蛋白，其序列与Ole e 1样蛋白家族的已知成员具有27% - 45%的同源性。77%对藜草花粉过敏患者的血清对Che a 1有反应。对Che a 1和油橄榄花粉主要过敏原Ole e 1的IgE反应性之间未发现相关性，且两种过敏原均显示出低水平（尽管可检测到）的IgE和IgG交叉反应性。
Che a 1是藜草花粉的一种相关过敏原，在结构上与Ole e 1样蛋白家族相关，但在其多肽序列上存在显著差异，这可能解释了其不同的抗原行为和有限的交叉反应性。
