Tsai P S, Uchima F D, Hamamoto S T, Bern H A
Department of Integrative Biology, University of California, Berkeley 94720.
In Vitro Cell Dev Biol. 1991 Jun;27A(6):461-8. doi: 10.1007/BF02631145.
Mouse vaginal epithelial cells were isolated from intact 21-day-old BALB/cCrgl mice and cultured in a serum-free medium (SF20: basal medium supplemented with insulin, epidermal growth factor, transferrin, and bovine serum albumin--fraction V) to examine the proliferation, differentiation, and specificity of estrogen-induced growth retardation in vitro. Histologic and ultrastructural studies showed that vaginal epithelial cells undergo differentiative changes in vitro in the absence of estrogen, and that these changes are similar to those induced in vivo by estrogen. Addition of 17 beta-estradiol inhibited cellular proliferation in a dose-dependent manner. Whereas other estrane derivatives (17 alpha-estradiol and estriol) also significantly retarded cellular proliferation, cholesterol, testosterone, and progesterone had no effect. Keoxifene, an antiestrogen, significantly reversed estrogen-induced growth inhibition, resulting in proliferation of estrogen-treated cells equivalent to that of the untreated control. The results suggest that both proliferation and differentiation of prepubertal mouse vaginal epithelial cells in vitro are estrogen-independent, and that the growth inhibition is a specific estrogen-induced response.
从小鼠阴道上皮细胞分离自完整的21日龄BALB/cCrgl小鼠，并在无血清培养基（SF20：基础培养基补充胰岛素、表皮生长因子、转铁蛋白和牛血清白蛋白 - V组分）中培养，以研究雌激素诱导的生长迟缓在体外的增殖、分化和特异性。组织学和超微结构研究表明，阴道上皮细胞在体外无雌激素的情况下会发生分化变化，且这些变化与雌激素在体内诱导的变化相似。添加17β-雌二醇以剂量依赖性方式抑制细胞增殖。而其他雌激素衍生物（17α-雌二醇和雌三醇）也显著抑制细胞增殖，胆固醇、睾酮和孕酮则无作用。抗雌激素药物他莫昔芬显著逆转雌激素诱导的生长抑制，导致雌激素处理细胞的增殖与未处理对照相当。结果表明，青春期前小鼠阴道上皮细胞在体外的增殖和分化均不依赖雌激素，且生长抑制是雌激素诱导的特异性反应。
