Yoneda Kyoko, Tomimoto Ayako, Endo Hiroki, Iida Hiroshi, Sugiyama Michiko, Takahashi Hirokazu, Mawatari Hironori, Nozaki Yuichi, Fujita Koji, Yoneda Masato, Inamori Masahiko, Nakajima Noriko, Wada Koichiro, Nagashima Yoji, Nakagama Hitoshi, Uozaki Hitoshi, Fukayama Masashi, Nakajima Atsushi
Gastroenterology Division, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, Japan.
Oncol Rep. 2008 Sep;20(3):479-83.
Adiponectin is secreted by adipocytes and is a key hormone responsible for insulin sensitization. Recent studies have shown that plasma adiponectin is decreased in patients with breast, endometrial and gastric cancer. However, the effect of adiponectin on colorectal carcinogenesis is controversial. It is now well known that the adiponectin receptor exists in two isoforms, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). We examined the expression of the adiponectin receptors on normal colon mucosa and colon cancer tissues in a human study using real-time RT-PCR, Western blotting and immunohistochemical staining. Adiponectin receptors, AdipoR1/AdipoR2, were expressed in normal colon epithelial and colon cancer cells. Furthermore, laser microdissection was performed to confirm our results. These results suggest that adiponectin may exert some effects on normal colon epithelium or colon cancer cells directly through adiponectin receptors. Further studies are required to elucidate the function of the AdipoRs activated by adiponectin and the downstream mechanisms of AdipoRs in colon cancer cells.
脂联素由脂肪细胞分泌，是一种负责胰岛素增敏作用的关键激素。最近的研究表明，乳腺癌、子宫内膜癌和胃癌患者的血浆脂联素水平降低。然而，脂联素对结直肠癌发生的影响存在争议。现在已知脂联素受体存在两种异构体，即脂联素受体1（AdipoR1）和脂联素受体2（AdipoR2）。我们在一项人体研究中，使用实时逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和免疫组织化学染色，检测了正常结肠黏膜和结肠癌组织中脂联素受体的表达。脂联素受体AdipoR1/AdipoR2在正常结肠上皮细胞和结肠癌细胞中均有表达。此外，还进行了激光显微切割以证实我们的结果。这些结果表明，脂联素可能通过脂联素受体直接对正常结肠上皮或结肠癌细胞发挥某些作用。需要进一步研究以阐明脂联素激活的脂联素受体的功能以及结肠癌细胞中脂联素受体的下游机制。
