Suppr超能文献

大肠杆菌DNA聚合酶IV对链间交联中间体的复制绕过

Replication bypass of interstrand cross-link intermediates by Escherichia coli DNA polymerase IV.

作者信息

Kumari Anuradha, Minko Irina G, Harbut Michael B, Finkel Steven E, Goodman Myron F, Lloyd R Stephen

机构信息

Center for Research on Occupational and Environmental Toxicology and the Department of Molecular and Medical Genetics, Oregon Health & Science University, Portland, Oregon 97239-3098.

Molecular and Computational Biology Program, Department of Biological Sciences, Los Angeles, California 90089-2910.

出版信息

J Biol Chem. 2008 Oct 10;283(41):27433-27437. doi: 10.1074/jbc.M801237200. Epub 2008 Aug 11.

Abstract

Repair of interstrand DNA cross-links (ICLs) in Escherichia coli can occur through a combination of nucleotide excision repair (NER) and homologous recombination. However, an alternative mechanism has been proposed in which repair is initiated by NER followed by translesion DNA synthesis (TLS) and completed through another round of NER. Using site-specifically modified oligodeoxynucleotides that serve as a model for potential repair intermediates following incision by E. coli NER proteins, the ability of E. coli DNA polymerases (pol) II and IV to catalyze TLS past N(2)-N(2)-guanine ICLs was determined. No biochemical evidence was found suggesting that pol II could bypass these lesions. In contrast, pol IV could catalyze TLS when the nucleotides that are 5' to the cross-link were removed. The efficiency of TLS was further increased when the nucleotides 3' to the cross-linked site were also removed. The correct nucleotide, C, was preferentially incorporated opposite the lesion. When E. coli cells were transformed with a vector carrying a site-specific N(2)-N(2)-guanine ICL, the transformation efficiency of a pol II-deficient strain was indistinguishable from that of the wild type. However, the ability to replicate the modified vector DNA was nearly abolished in a pol IV-deficient strain. These data strongly suggest that pol IV is responsible for TLS past N(2)-N(2)-guanine ICLs.

摘要

大肠杆菌中链间DNA交联(ICL)的修复可通过核苷酸切除修复(NER)和同源重组相结合的方式进行。然而,有人提出了另一种机制,即修复由NER启动,随后进行跨损伤DNA合成(TLS),并通过另一轮NER完成。使用位点特异性修饰的寡脱氧核苷酸作为大肠杆菌NER蛋白切割后潜在修复中间体的模型,测定了大肠杆菌DNA聚合酶(pol)II和IV催化TLS越过N(2)-N(2)-鸟嘌呤ICL的能力。未发现生化证据表明pol II可以绕过这些损伤。相反,当交联位点5'端的核苷酸被去除时,pol IV可以催化TLS。当交联位点3'端的核苷酸也被去除时,TLS的效率进一步提高。正确的核苷酸C优先掺入损伤对面。当用携带位点特异性N(2)-N(2)-鸟嘌呤ICL的载体转化大肠杆菌细胞时,pol II缺陷菌株的转化效率与野生型菌株无异。然而,在pol IV缺陷菌株中,复制修饰载体DNA的能力几乎丧失。这些数据强烈表明,pol IV负责越过N(2)-N(2)-鸟嘌呤ICL的TLS。

相似文献

1
Replication bypass of interstrand cross-link intermediates by Escherichia coli DNA polymerase IV.
J Biol Chem. 2008 Oct 10;283(41):27433-27437. doi: 10.1074/jbc.M801237200. Epub 2008 Aug 11.
2
A Comprehensive View of Translesion Synthesis in Escherichia coli.
Microbiol Mol Biol Rev. 2020 Jun 17;84(3). doi: 10.1128/MMBR.00002-20. Print 2020 Aug 19.
4
Replication bypass of the acrolein-mediated deoxyguanine DNA-peptide cross-links by DNA polymerases of the DinB family.
Chem Res Toxicol. 2008 Oct;21(10):1983-90. doi: 10.1021/tx800174a. Epub 2008 Sep 13.
5
Replication bypass of N2-deoxyguanosine interstrand cross-links by human DNA polymerases η and ι.
Chem Res Toxicol. 2012 Mar 19;25(3):755-62. doi: 10.1021/tx300011w. Epub 2012 Feb 29.
6
Role for DNA polymerase kappa in the processing of N2-N2-guanine interstrand cross-links.
J Biol Chem. 2008 Jun 20;283(25):17075-82. doi: 10.1074/jbc.M801238200. Epub 2008 Apr 22.
8
Mutagenic Bypass of an Oxidized Abasic Lesion-Induced DNA Interstrand Cross-Link Analogue by Human Translesion Synthesis DNA Polymerases.
Biochemistry. 2015 Dec 22;54(50):7409-22. doi: 10.1021/acs.biochem.5b01027. Epub 2015 Dec 14.
9
Role of high-fidelity Escherichia coli DNA polymerase I in replication bypass of a deoxyadenosine DNA-peptide cross-link.
J Bacteriol. 2011 Aug;193(15):3815-21. doi: 10.1128/JB.01550-10. Epub 2011 May 27.
10
Mechanisms employed by Escherichia coli to prevent ribonucleotide incorporation into genomic DNA by Pol V.
PLoS Genet. 2012;8(11):e1003030. doi: 10.1371/journal.pgen.1003030. Epub 2012 Nov 8.

引用本文的文献

3
Specialised DNA polymerases in Escherichia coli: roles within multiple pathways.
Curr Genet. 2018 Dec;64(6):1189-1196. doi: 10.1007/s00294-018-0840-x. Epub 2018 Apr 26.
4
DNA polymerase IV primarily operates outside of DNA replication forks in Escherichia coli.
PLoS Genet. 2018 Jan 19;14(1):e1007161. doi: 10.1371/journal.pgen.1007161. eCollection 2018 Jan.
5
O-2'-Deoxyguanosine-butylene-O-2'-deoxyguanosine DNA Interstrand Cross-Links Are Replication-Blocking and Mutagenic DNA Lesions.
Chem Res Toxicol. 2016 Nov 21;29(11):1872-1882. doi: 10.1021/acs.chemrestox.6b00278. Epub 2016 Nov 4.
6
Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli.
J Bacteriol. 2016 Oct 21;198(22):3099-3108. doi: 10.1128/JB.00509-16. Print 2016 Nov 15.
7
Translesion DNA Synthesis.
EcoSal Plus. 2012 Nov;5(1). doi: 10.1128/ecosalplus.7.2.2.
9
DNA polymerase IV mediates efficient and quick recovery of replication forks stalled at N2-dG adducts.
Nucleic Acids Res. 2014 Jul;42(13):8461-72. doi: 10.1093/nar/gku547. Epub 2014 Jun 23.
10
Multiple strategies for translesion synthesis in bacteria.
Cells. 2012 Oct 15;1(4):799-831. doi: 10.3390/cells1040799.

本文引用的文献

1
Role for DNA polymerase kappa in the processing of N2-N2-guanine interstrand cross-links.
J Biol Chem. 2008 Jun 20;283(25):17075-82. doi: 10.1074/jbc.M801238200. Epub 2008 Apr 22.
2
Formation and repair of interstrand cross-links in DNA.
Chem Rev. 2006 Feb;106(2):277-301. doi: 10.1021/cr040478b.
4
Evidence for Escherichia coli polymerase II mutagenic bypass of intrastrand DNA crosslinks.
DNA Repair (Amst). 2005 Dec 8;4(12):1374-80. doi: 10.1016/j.dnarep.2005.08.011. Epub 2005 Oct 27.
5
DNA interchain cross-links formed by acrolein and crotonaldehyde.
J Am Chem Soc. 2003 Jan 8;125(1):50-61. doi: 10.1021/ja020778f.
8
Fidelity of Escherichia coli DNA polymerase IV. Preferential generation of small deletion mutations by dNTP-stabilized misalignment.
J Biol Chem. 2002 Sep 13;277(37):34198-207. doi: 10.1074/jbc.M204826200. Epub 2002 Jul 3.
9
SOS-induced DNA polymerases enhance long-term survival and evolutionary fitness.
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8737-41. doi: 10.1073/pnas.092269199. Epub 2002 Jun 11.
10
Error prone translesion synthesis past gamma-hydroxypropano deoxyguanosine, the primary acrolein-derived adduct in mammalian cells.
J Biol Chem. 2002 May 24;277(21):18257-65. doi: 10.1074/jbc.M112419200. Epub 2002 Mar 11.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验