Mukherjee R M, Bhol K C, Mehra S, Maitra T K, Jalan K N
Kothari Centre of Gastroenterology, Kothari Medical Centre, Calcutta, India.
J Diarrhoeal Dis Res. 1991 Mar;9(1):11-5.
Entamoeba histolytica isolates, obtained mainly from asymptomatic human cyst passers, were tested for their in vitro erythrophagocytic activity and lesion-forming ability in hamster liver. Simultaneous zymodeme typing of the isolates showed that, along with the pathogenic zymodemes, a number of isolates having nonpathogenic zymodemes also produced liver lesions in hamsters and engulfed higher numbers of RBCs. The observed positive correlation between the rate of erythrophagocytosis of an E. histolytica trophozoite and its degree of lesion-producing capacity in hamster liver suggest that the erythrophagocytosis assay in conjunction with the hamster liver inoculation test, can be useful in the identification of virulent E. histolytica isolates.
主要从无症状排包囊者体内分离得到的溶组织内阿米巴菌株，对其体外红细胞吞噬活性以及在仓鼠肝脏中形成病变的能力进行了检测。同时对这些菌株进行酶谱分型，结果显示，除了致病酶谱型外，一些具有非致病酶谱型的菌株在仓鼠体内也会产生肝脏病变，并且吞噬了更多数量的红细胞。观察到溶组织内阿米巴滋养体的红细胞吞噬率与其在仓鼠肝脏中产生病变的能力之间呈正相关，这表明红细胞吞噬试验结合仓鼠肝脏接种试验，可用于鉴定溶组织内阿米巴的强毒株。
