Wang F
Department of Ophthalmology, University Hospital Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.
Ophthalmologica. 2008;222(6):369-72. doi: 10.1159/000151247. Epub 2008 Aug 12.
A mouse model of combined UVA/riboflavin irradiation to eliminate stromal cells and other antigen-presenting cells in the cornea provides the basis for a probably low risk of corneal transplantation.
After abrasion of the epithelium, the central corneas of mouse eyes were treated with UVA/riboflavin in vitro. Histological studies of hematoxylin-eosin and immunohistochemical staining with caspase 3 were performed. Dissected mouse corneas were analyzed by Western blot.
Apoptotic cells were shown on the central corneal stroma; a cell-free zone was displayed in the cornea. Numbers of dead cells increased according to cultivation time. However, the endothelium survived due to the adjustment of the irradiation dose.
A cell-free zone in the stroma of the mouse cornea was produced by UVA/riboflavin irradiation in vitro. The technique makes possible to prevent or reduce immunological reactions and the risk of graft rejection by pretreatment of the donor cornea, ultimately prolonging graft survival.
联合紫外线A（UVA）/核黄素照射以清除角膜基质细胞和其他抗原呈递细胞的小鼠模型为角膜移植可能的低风险提供了基础。
在角膜上皮擦伤后，对小鼠眼的中央角膜进行体外UVA/核黄素处理。进行苏木精-伊红组织学研究和半胱天冬酶3免疫组化染色。对解剖的小鼠角膜进行蛋白质印迹分析。
在中央角膜基质上显示出凋亡细胞；角膜中出现无细胞区。死细胞数量随培养时间增加。然而，由于调整了照射剂量，内皮细胞得以存活。
体外UVA/核黄素照射在小鼠角膜基质中产生了无细胞区。该技术通过对供体角膜进行预处理，使得预防或减少免疫反应及移植物排斥风险成为可能，最终延长移植物存活时间。
