Pupi A, De Cristofaro M T, Bacciottini L, Antoniucci D, Formiconi A R, Mascalchi M, Meldolesi U
Dipartimento di Fisiopatologia Clinica, U.O. Cardiologia, USL 10/D Firenze, Italy.
J Nucl Med. 1991 Aug;32(8):1501-6.
Arterial radioactivity content after the intravenous administration of HMPAO in seven human subjects was analyzed. Arterial sampling of 99mTc-HMPAO was performed on each subject over a 25-min period postinjection. The lipophilic fraction of the tracer present in the blood was rapidly extracted with octanol. An analysis of the time course of the extracted and nonextracted octanol fractions was performed in order to calculate the arterial input of the tracer available for brain extraction. HMPAO net regional brain clearances were then calculated and compared with rCBF values obtained in the same patients using 99mTc-microspheres injected into the left ventricle of the heart. HMPAO brain clearances were 0.41 +/- 0.01 and 0.27 +/- 0.01 ml/min/g for grey and white matter, respectively. Linear regression analysis was performed and the following result was obtained: clearance (HMPAO) = 0.07 + 0.43 . rCBF with a high significance (p less than 0.001). This equation can be used for the transformation of HMPAO clearances into rCBF values. Our study demonstrates that by using HMPAO and SPECT it is possible to obtain a quantitative estimate of rCBF in humans.
分析了7名受试者静脉注射HMPAO后的动脉放射性含量。在注射后25分钟内对每名受试者进行99mTc-HMPAO的动脉采样。血液中示踪剂的亲脂部分用辛醇快速提取。对提取和未提取的辛醇部分的时间进程进行分析，以计算可用于脑提取的示踪剂的动脉输入量。然后计算HMPAO的脑净区域清除率，并与使用注入心脏左心室的99mTc-微球在同一患者中获得的rCBF值进行比较。灰质和白质的HMPAO脑清除率分别为0.41±0.01和0.27±0.01 ml/min/g。进行线性回归分析，得到以下结果：清除率（HMPAO）=0.07 + 0.43×rCBF，具有高度显著性（p＜0.001）。该方程可用于将HMPAO清除率转换为rCBF值。我们的研究表明，通过使用HMPAO和SPECT可以对人体的rCBF进行定量估计。
