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2
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Oligomerization of neuropeptide Y (NPY) Y2 receptors in CHO cells depends on functional pertussis toxin-sensitive G-proteins.神经肽Y（NPY）Y2受体在CHO细胞中的寡聚化取决于对百日咳毒素敏感的功能性G蛋白。
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引用本文的文献

1
Surface masking shapes the traffic of the neuropeptide Y Y2 receptor.表面掩蔽塑造了神经肽 Y Y2 受体的运输。
Peptides. 2012 Sep;37(1):40-8. doi: 10.1016/j.peptides.2012.06.008. Epub 2012 Jun 23.
2
Chronic stress alters neuropeptide Y signaling in the bed nucleus of the stria terminalis in DBA/2J but not C57BL/6J mice.慢性应激改变 DBA/2J 小鼠但不改变 C57BL/6J 小鼠终纹床核内神经肽 Y 信号。
Neuropharmacology. 2012 Mar;62(4):1777-86. doi: 10.1016/j.neuropharm.2011.12.002. Epub 2011 Dec 9.

本文引用的文献

1
Pertussis toxin induces parallel loss of neuropeptide Y Y1 receptor dimers and Gi alpha subunit function in CHO cells.百日咳毒素可诱导CHO细胞中神经肽Y Y1受体二聚体和Giα亚基功能同时丧失。
Eur J Pharmacol. 2008 Jan 28;579(1-3):13-25. doi: 10.1016/j.ejphar.2007.10.002. Epub 2007 Oct 11.
2
Neuropeptide Y Y2 receptor in health and disease.健康与疾病中的神经肽Y Y2受体
Br J Pharmacol. 2008 Feb;153(3):420-31. doi: 10.1038/sj.bjp.0707445. Epub 2007 Sep 10.
3
Oligomerization of neuropeptide Y (NPY) Y2 receptors in CHO cells depends on functional pertussis toxin-sensitive G-proteins.神经肽Y（NPY）Y2受体在CHO细胞中的寡聚化取决于对百日咳毒素敏感的功能性G蛋白。
Regul Pept. 2007 Dec 4;144(1-3):72-81. doi: 10.1016/j.regpep.2007.06.004. Epub 2007 Jun 21.
4
Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome.神经肽Y在外周直接作用于脂肪组织，介导应激诱导的肥胖和代谢综合征。
Nat Med. 2007 Jul;13(7):803-11. doi: 10.1038/nm1611. Epub 2007 Jul 1.
5
Neuropeptide Y (NPY) cleaving enzymes: structural and functional homologues of dipeptidyl peptidase 4.神经肽Y（NPY）裂解酶：二肽基肽酶4的结构和功能同源物。
Peptides. 2007 Feb;28(2):257-68. doi: 10.1016/j.peptides.2006.09.027. Epub 2007 Jan 16.
6
Tracking of human Y receptors in living cells--a fluorescence approach.
Peptides. 2007 Feb;28(2):226-34. doi: 10.1016/j.peptides.2006.08.042. Epub 2007 Jan 4.
7
Self-regulation of agonist activity at the Y receptors.Y受体激动剂活性的自我调节。
Peptides. 2007 Feb;28(2):203-13. doi: 10.1016/j.peptides.2006.07.032. Epub 2006 Dec 27.
8
Parallel inactivation of Y2 receptor and G-proteins in CHO cells by pertussis toxin.百日咳毒素对CHO细胞中Y2受体和G蛋白的平行失活作用。
Regul Pept. 2007 Mar 1;139(1-3):128-35. doi: 10.1016/j.regpep.2006.10.014. Epub 2006 Dec 18.
9
Phosphate stabilization of intermolecular interactions.分子间相互作用的磷酸盐稳定作用。
J Proteome Res. 2006 Jan;5(1):122-6. doi: 10.1021/pr0503578.
10
Internalization of cloned pancreatic polypeptide receptors is accelerated by all types of Y4 agonists.所有类型的Y4激动剂均可加速克隆的胰多肽受体的内化。
Regul Pept. 2005 Dec 15;132(1-3):91-101. doi: 10.1016/j.regpep.2005.09.008. Epub 2005 Oct 4.

N端天冬氨酸在神经肽Y Y2受体内化中的重要性。

Importance of a N-terminal aspartate in the internalization of the neuropeptide Y Y2 receptor.

作者信息

Parker Steven L, Parker Michael S, Wong Ying Y, Sah Renu, Balasubramaniam Ambikaipakan, Sallee Floyd

机构信息

Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

出版信息

Eur J Pharmacol. 2008 Oct 10;594(1-3):26-31. doi: 10.1016/j.ejphar.2008.07.038. Epub 2008 Jul 30.

DOI:10.1016/j.ejphar.2008.07.038
PMID:18700141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2579264/
Abstract

With human neuropeptide Y Y2 receptor expressed in the Chinese hamster ovary (CHO) cells, the Asp35Ala mutation, and especially the change of Pro34Asp35 to Ala34Ala35, decrease the compartmentalization and strongly accelerate internalization of the receptor. These changes are not associated with alterations in agonist affinity, G-protein interaction, dimerization, or level of expression of the mutated receptors relative to the wildtype receptor. The proline-flanked aspartate in the N-terminal extracellular segment of the neuropeptide Y Y2 receptor thus apparently has a large role in anchoring and compartmentalization of the receptor. However, the Pro34Ala mutation does not significantly affect the embedding and cycling of the receptor.

摘要

在中国仓鼠卵巢（CHO）细胞中表达的人神经肽Y Y2受体，Asp35Ala突变，尤其是Pro34Asp35变为Ala34Ala35，会减少受体的区室化并强烈加速其内化。这些变化与激动剂亲和力、G蛋白相互作用、二聚化或突变受体相对于野生型受体的表达水平改变无关。因此，神经肽Y Y2受体N端细胞外段中脯氨酸侧翼的天冬氨酸显然在受体的锚定和区室化中起很大作用。然而，Pro34Ala突变对受体的嵌入和循环没有显著影响。