Wu Ma-li, Ye Gong-yin, Zhu Jia-ying, Chen Xue-xin, Hu Cui
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
J Invertebr Pathol. 2008 Oct;99(2):186-91. doi: 10.1016/j.jip.2008.07.005. Epub 2008 Jul 25.
In hymenopteran parasitoids devoid of symbiotic viruses, venom proteins appear to play a major role in host immune suppression and host regulation. Not much is known about the active components of venom proteins in these parasitoids, especially those that have the functions involved in the suppression of host cellular immunity. Here, we report the isolation and characterization of a venom protein Vn.11 with 24.1 kDa in size from Pteromalus puparum, a pupa-specific endoparasitoid of Pieris rapae. The Vn.11 venom protein is isolated with the combination of ammonium sulfate precipitation and anion exchange chromatography, and its purity is verified using SDS-PAGE analysis. Like crude venom, the Vn.11 venom protein significantly inhibits the spreading behavior and encapsulation ability of host hemocytes in vitro. It is suggested that this protein is an actual component of P. puparum crude venom as host cellular-immune suppressive factor.
在没有共生病毒的膜翅目寄生蜂中，毒液蛋白似乎在宿主免疫抑制和宿主调节中起主要作用。对于这些寄生蜂中毒液蛋白的活性成分，尤其是那些具有抑制宿主细胞免疫功能的成分，人们了解得并不多。在此，我们报告了从菜粉蝶蛹特异性内寄生蜂蝶蛹金小蜂中分离出的一种大小为24.1 kDa的毒液蛋白Vn.11及其特性。通过硫酸铵沉淀和阴离子交换色谱相结合的方法分离出Vn.11毒液蛋白，并使用SDS-PAGE分析验证其纯度。与粗毒液一样，Vn.11毒液蛋白在体外能显著抑制宿主血细胞的铺展行为和包囊能力。据推测，该蛋白是蝶蛹金小蜂粗毒液中作为宿主细胞免疫抑制因子的实际成分。
