Suppr超能文献

姬蜂蛹毒液通过降低其清道夫受体基因的表达来损害宿主的细胞免疫反应。

Pteromalus puparum venom impairs host cellular immune responses by decreasing expression of its scavenger receptor gene.

机构信息

State Key Laboratory of Rice Biology & Key Laboratory of Molecular Biology of Crop Pathogens and Insects of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China.

出版信息

Insect Biochem Mol Biol. 2011 Nov;41(11):852-62. doi: 10.1016/j.ibmb.2011.07.001. Epub 2011 Jul 22.

Abstract

Insect host/parasitoid interactions are co-evolved systems in which host defenses are balanced by parasitoid mechanisms to disable or hide from host immune effectors. Although there is a rich literature on these systems, parasitoid immune-disabling mechanisms have not been fully elucidated. Here we report on a newly discovered immune-disabling mechanism in the Pieris rapae/Pteromalus puparum host/parasitoid system. Because venom injections and parasitization suppresses host phagocytosis, we turned attention to the P. rapae scavenger receptor (Pr-SR), posing the hypothesis that P. puparum venom suppresses expression of the host Pr-SR gene. To test our hypothesis, we cloned a full-length cDNA of the Pr-SR. Multiple sequences alignment showed the deduced amino acid sequence of Pr-SR is similar to scavenger receptors of other lepidopterans. Bacterial and bead injections induced Pr-SR mRNA and protein expression, which peaked at 4h post-bead injection. Venom injection inhibited Pr-SR expression. Pr-SR was specifically expressed in granulocytes compared to plasmatocytes. We localized the Pr-SR protein in cytoplasm and cellular membrane, with no evidence of secretion into host plasma. Double-strand RNA designed to Pr-SR mRNA silenced expression of Pr-SR and significantly impaired host phagocytosis and encapsulation reactions. Venom injections similarly silenced Pr-SR expression during the first 8h post-treatment, after which the silencing effects gradually abated. We infer from these findings that one mechanism of impairing P. rapae hemocytic immune reactions is by silencing expression of Pr-SR.

摘要

昆虫宿主/寄生蜂相互作用是共同进化的系统,在这个系统中,宿主防御受到寄生蜂机制的平衡,以使其失活或躲避宿主免疫效应物。尽管有丰富的关于这些系统的文献,但寄生蜂的免疫失活机制尚未完全阐明。在这里,我们报告了在小菜蛾/蛹期姬蜂宿主/寄生蜂系统中发现的一种新的免疫失活机制。由于毒液注射和寄生会抑制宿主吞噬作用,我们将注意力转向小菜蛾的清道夫受体(Pr-SR),提出假设认为蛹期姬蜂的毒液抑制了宿主 Pr-SR 基因的表达。为了验证我们的假设,我们克隆了 Pr-SR 的全长 cDNA。多重序列比对显示,Pr-SR 的推导氨基酸序列与其他鳞翅目昆虫的清道夫受体相似。细菌和珠粒注射诱导 Pr-SR mRNA 和蛋白表达,在珠粒注射后 4 小时达到峰值。毒液注射抑制了 Pr-SR 的表达。Pr-SR 在粒细胞中的表达特异性高于浆细胞。我们将 Pr-SR 蛋白定位于细胞质和细胞膜中,没有证据表明其分泌到宿主血浆中。针对 Pr-SR mRNA 的双链 RNA 沉默了 Pr-SR 的表达,并显著损害了宿主的吞噬和包被反应。毒液注射在处理后的前 8 小时内同样沉默了 Pr-SR 的表达,之后沉默效应逐渐减弱。根据这些发现,我们推断,损害小菜蛾血细胞免疫反应的一种机制是通过沉默 Pr-SR 的表达。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验